Overview

  • Product nameAnti-IBRDC2 antibody
    See all IBRDC2 primary antibodies
  • Description
    Rabbit polyclonal to IBRDC2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 215-264 (KHTFCWYCLQ NLDNDIFLRH YDKGPCRNKL GHSRASVMWN RTQVVGILVG) of Human IBRDC2 (NP_877434).

  • Positive control
    • ACHN cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab106327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.
  • Tissue specificityBroadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the RBR family. RNF144 subfamily.
    Contains 1 IBR-type zinc finger.
    Contains 2 RING-type zinc fingers.
  • DomainThe RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme (By similarity). The transmembrane domain is essential for translocation to the mitochondria upon induction of apoptosis.
  • Post-translational
    modifications
    Auto-ubiquitinated.
  • Cellular localizationMitochondrion membrane. Cytoplasm. Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA528A10.3 antibody
    • E3 ubiquitin protein ligase RNF144B antibody
    • E3 ubiquitin-protein ligase RNF144B antibody
    • IBR domain containing 2 antibody
    • IBR domain containing protein 2 antibody
    • IBR domain-containing protein 2 antibody
    • IBRDC 2 antibody
    • IBRDC2 antibody
    • KIAA0161 antibody
    • MGC71786 antibody
    • OTTHUMP00000016079 antibody
    • OTTHUMP00000016080 antibody
    • OTTHUMP00000039360 antibody
    • p53 inducible RING finger protein antibody
    • p53-inducible RING finger protein antibody
    • p53RFP antibody
    • PIR2 antibody
    • R144B_HUMAN antibody
    • Ring finger 144B antibody
    • RING finger protein 144B antibody
    • RNF 144B antibody
    • RNF144B antibody
    see all

Anti-IBRDC2 antibody images

  • Anti-IBRDC2 antibody (ab106327) at 1 µg/ml + ACHN cell lysate at 10 µg

    Predicted band size : 34 kDa

References for Anti-IBRDC2 antibody (ab106327)

ab106327 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"