Overview

  • Product nameAnti-IBTK antibody
  • Description
    Rabbit polyclonal to IBTK
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 589-617 of Human IBTK (NP_056340.2)

  • Positive control
    • MDA-MB435 cell line lysates Human lymph tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG

Associated products

Applications

Our Abpromise guarantee covers the use of ab107852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 151 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionActs as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. Down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription.
  • Tissue specificityExpressed in DeFew, 293T, Hela and in Jurkat, MC3 and NB4 lymphoid cells (at protein level). Isoform 1 is the predominant isoform expressed in all examined tissues and cell lines. Highly expressed in hemopoietic tissues (fetal liver, spleen, lymph node, thymus, peripheral blood leukocytes and bone marrow). Weakly or not expressed in other tissues.
  • Sequence similaritiesContains 3 ANK repeats.
    Contains 2 BTB (POZ) domains.
    Contains 3 RCC1 repeats.
  • Cellular localizationNucleus and Cytoplasm. Membrane. Translocates to the plasma membrane upon IgM stimulation.
  • Information by UniProt
  • Database links
  • Alternative names
    • BTKI antibody
    • IBtk antibody
    • IBTK_HUMAN antibody
    • inhibitor of Bruton agammaglobulinemia tyrosine kinase antibody
    • Inhibitor of Bruton tyrosine kinase antibody
    see all

Anti-IBTK antibody images

  • Anti-IBTK antibody (ab107852) at 1/100 dilution + MDA-MB435 cell line lysates at 35 µg

    Predicted band size : 151 kDa
  • Immunohistochemistry analysis in formalin fixed and paraffin embedded Human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. ab107852 at 1/50.

References for Anti-IBTK antibody (ab107852)

ab107852 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Defew B-cell Lymphoma)
Total protein in input 1000 µg
Immuno-precipitation step Other - TrueBlot anti-rabbit beads
Specification Defew B-cell Lymphoma
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Submitted May 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"