Overview

  • Product name
    Anti-IBTK antibody
  • Description
    Rabbit polyclonal to IBTK
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 589-617 of Human IBTK (NP_056340.2)

  • Positive control
    • MDA-MB435 cell line lysates Human lymph tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab107852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 151 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Acts as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. Down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription.
  • Tissue specificity
    Expressed in DeFew, 293T, Hela and in Jurkat, MC3 and NB4 lymphoid cells (at protein level). Isoform 1 is the predominant isoform expressed in all examined tissues and cell lines. Highly expressed in hemopoietic tissues (fetal liver, spleen, lymph node, thymus, peripheral blood leukocytes and bone marrow). Weakly or not expressed in other tissues.
  • Sequence similarities
    Contains 3 ANK repeats.
    Contains 2 BTB (POZ) domains.
    Contains 3 RCC1 repeats.
  • Cellular localization
    Nucleus and Cytoplasm. Membrane. Translocates to the plasma membrane upon IgM stimulation.
  • Information by UniProt
  • Database links
  • Alternative names
    • BTKI antibody
    • IBtk antibody
    • IBTK_HUMAN antibody
    • inhibitor of Bruton agammaglobulinemia tyrosine kinase antibody
    • Inhibitor of Bruton tyrosine kinase antibody
    see all

Images

  • Anti-IBTK antibody (ab107852) at 1/100 dilution + MDA-MB435 cell line lysates at 35 µg

    Predicted band size : 151 kDa
  • Immunohistochemistry analysis in formalin fixed and paraffin embedded Human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. ab107852 at 1/50.

References

ab107852 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Defew B-cell Lymphoma)
Total protein in input
1000 µg
Immuno-precipitation step
Other - TrueBlot anti-rabbit beads
Specification
Defew B-cell Lymphoma
Username

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Verified customer

Submitted May 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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