Overview

  • Product nameAnti-ICAD antibody
    See all ICAD primary antibodies
  • Description
    Rabbit polyclonal to ICAD
  • SpecificityDetects endogenous levels of total ICAD protein.
  • Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Human) from an internal region

  • Positive control
    • Human breast carcinoma tissue 293, Jurkat cell and HeLa cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab65129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

Target

  • FunctionInhibitor of the caspase-activated DNase (DFF40).
  • Sequence similaritiesContains 1 CIDE-N domain.
  • Post-translational
    modifications
    Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • A330085O09Rik antibody
    • Caspase activated deoxyribonuclease inhibitor short form antibody
    • DFF 1 antibody
    • DFF 45 antibody
    • DFF alpha antibody
    • DFF-45 antibody
    • DFF1 antibody
    • DFF35 antibody
    • DFF45 antibody
    • DFFA antibody
    • Dffa DNA fragmentation factor, alpha subunit antibody
    • DFFA_HUMAN antibody
    • DNA fragmentation factor 45 kDa subunit antibody
    • DNA Fragmentation Factor Alpha Subunit antibody
    • DNA fragmentation factor subunit alpha antibody
    • DNA fragmentation factor, 45 kD, alpha subunit antibody
    • DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1 antibody
    • DNA fragmentation factor, 45kDa, alpha polypeptide antibody
    • DNA fragmentation factor, alpha subunit antibody
    • DNAation factor 45 kDa subunit antibody
    • H13 antibody
    • ICAD antibody
    • ICAD L antibody
    • ICAD S antibody
    • Inhibitor of CAD antibody
    • Inhibitor of Caspase Activated DNase antibody
    • MGC143066 antibody
    • OTTHUMP00000001903 antibody
    • OTTHUMP00000001904 antibody
    • RP23 121D17.3 antibody
    see all

Anti-ICAD antibody images

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelled with ab65129 at 1/50 dilution. The right hand panel was also treated with immunising peptide.
  • All lanes : Anti-ICAD antibody (ab65129) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : Jurkat cell extract
    Lane 3 : HeLa cell extract
    Lane 4 : HeLa cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ICAD antibody (ab65129)

ab65129 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"