Overview

  • Product name
  • Description
    Rabbit polyclonal to ICAD
  • Tested applications
    Suitable for: ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    KASPPGDLQNPKRARQDPT

    , corresponding to amino acids 313/331 of Human ICAD (Peptide available as ab8441.)

  • Positive control
    • HeLa whole cell lysate A 45 kDa band can be detected innon-apoptotic cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab8409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent dilution.
WB 1/1000 - 1/2000. Detects a band of approximately 45 kDa.Can be blocked with Human ICAD peptide (ab8441).

Target

  • Function
    Inhibitor of the caspase-activated DNase (DFF40).
  • Sequence similarities
    Contains 1 CIDE-N domain.
  • Post-translational
    modifications
    Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • A330085O09Rik antibody
    • Caspase activated deoxyribonuclease inhibitor short form antibody
    • DFF 1 antibody
    • DFF 45 antibody
    • DFF alpha antibody
    • DFF-45 antibody
    • DFF1 antibody
    • DFF35 antibody
    • DFF45 antibody
    • DFFA antibody
    • Dffa DNA fragmentation factor, alpha subunit antibody
    • DFFA_HUMAN antibody
    • DNA fragmentation factor 45 kDa subunit antibody
    • DNA Fragmentation Factor Alpha Subunit antibody
    • DNA fragmentation factor subunit alpha antibody
    • DNA fragmentation factor, 45 kD, alpha subunit antibody
    • DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1 antibody
    • DNA fragmentation factor, 45kDa, alpha polypeptide antibody
    • DNA fragmentation factor, alpha subunit antibody
    • DNAation factor 45 kDa subunit antibody
    • H13 antibody
    • ICAD antibody
    • ICAD L antibody
    • ICAD S antibody
    • Inhibitor of CAD antibody
    • Inhibitor of Caspase Activated DNase antibody
    • MGC143066 antibody
    • OTTHUMP00000001903 antibody
    • OTTHUMP00000001904 antibody
    • RP23 121D17.3 antibody
    see all

Images

  • All lanes : Anti-ICAD antibody (ab8409) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : Jurkat whole cell lysate
    Lane 3 : A431 whole cell lysate
    Lane 4 : K562 whole cell lysate


    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • ab8409 at 5µg/ml staining ICAD in Hela cells by ICC/IF

References

This product has been referenced in:
  • Borchert GM  et al. Adenosine deamination in human transcripts generates novel microRNA binding sites. Hum Mol Genet 18:4801-7 (2009). Read more (PubMed: 19776031) »
  • Zhu P  et al. Granzyme A, which causes single-stranded DNA damage, targets the double-strand break repair protein Ku70. EMBO Rep 7:431-7 (2006). Read more (PubMed: 16440001) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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