Overview

  • Product nameAnti-ICAD antibody
    See all ICAD primary antibodies
  • Description
    Rabbit polyclonal to ICAD
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to a region within the C terminal amino acids of Human ICAD (NP_998731.1).

  • Positive control
    • HeLa and 293T whole cell lysates

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab99374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 37 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionInhibitor of the caspase-activated DNase (DFF40).
  • Sequence similaritiesContains 1 CIDE-N domain.
  • Post-translational
    modifications
    Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • A330085O09Rik antibody
    • Caspase activated deoxyribonuclease inhibitor short form antibody
    • DFF 1 antibody
    • DFF 45 antibody
    • DFF alpha antibody
    • DFF-45 antibody
    • DFF1 antibody
    • DFF35 antibody
    • DFF45 antibody
    • DFFA antibody
    • Dffa DNA fragmentation factor, alpha subunit antibody
    • DFFA_HUMAN antibody
    • DNA fragmentation factor 45 kDa subunit antibody
    • DNA Fragmentation Factor Alpha Subunit antibody
    • DNA fragmentation factor subunit alpha antibody
    • DNA fragmentation factor, 45 kD, alpha subunit antibody
    • DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1 antibody
    • DNA fragmentation factor, 45kDa, alpha polypeptide antibody
    • DNA fragmentation factor, alpha subunit antibody
    • DNAation factor 45 kDa subunit antibody
    • H13 antibody
    • ICAD antibody
    • ICAD L antibody
    • ICAD S antibody
    • Inhibitor of CAD antibody
    • Inhibitor of Caspase Activated DNase antibody
    • MGC143066 antibody
    • OTTHUMP00000001903 antibody
    • OTTHUMP00000001904 antibody
    • RP23 121D17.3 antibody
    see all

Anti-ICAD antibody images

  • All lanes : Anti-ICAD antibody (ab99374) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 37 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99374 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane

    Western Blot: ICD antibody (ab99374) at 1 µg/ml

    developed using the ECL technique

    Exposure time : 10 seconds

    Predicted band size : 37 kDa

References for Anti-ICAD antibody (ab99374)

ab99374 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"