Overview

  • Product nameAnti-ICAD antibody [AF24G5]
    See all ICAD primary antibodies
  • Description
    Mouse monoclonal [AF24G5] to ICAD
  • Tested applicationsSuitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length ICAD protein (Human) purified from E.coli (His-ICAD)

  • Positive control
    • Hela, A431, HepG2, 293T and Jurkat cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab75514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    IP: 3µl/ 400µl lysate.
    WB: 1/2000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInhibitor of the caspase-activated DNase (DFF40).
    • Sequence similaritiesContains 1 CIDE-N domain.
    • Post-translational
      modifications
      Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • A330085O09Rik antibody
      • Caspase activated deoxyribonuclease inhibitor short form antibody
      • DFF 1 antibody
      • DFF 45 antibody
      • DFF alpha antibody
      • DFF-45 antibody
      • DFF1 antibody
      • DFF35 antibody
      • DFF45 antibody
      • DFFA antibody
      • Dffa DNA fragmentation factor, alpha subunit antibody
      • DFFA_HUMAN antibody
      • DNA fragmentation factor 45 kDa subunit antibody
      • DNA Fragmentation Factor Alpha Subunit antibody
      • DNA fragmentation factor subunit alpha antibody
      • DNA fragmentation factor, 45 kD, alpha subunit antibody
      • DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1 antibody
      • DNA fragmentation factor, 45kDa, alpha polypeptide antibody
      • DNA fragmentation factor, alpha subunit antibody
      • DNAation factor 45 kDa subunit antibody
      • H13 antibody
      • ICAD antibody
      • ICAD L antibody
      • ICAD S antibody
      • Inhibitor of CAD antibody
      • Inhibitor of Caspase Activated DNase antibody
      • MGC143066 antibody
      • OTTHUMP00000001903 antibody
      • OTTHUMP00000001904 antibody
      • RP23 121D17.3 antibody
      see all

    Anti-ICAD antibody [AF24G5] images

    • All lanes : Anti-ICAD antibody [AF24G5] (ab75514) at 1/2000 dilution

      Lane 1 : Hela cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : Jurkat cell lysate


      Predicted band size : 37 kDa
      Observed band size : 37 kDa

    References for Anti-ICAD antibody [AF24G5] (ab75514)

    ab75514 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab75514.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"