Overview

  • Product name
    Anti-ICAD antibody [AF24G5]
    See all ICAD primary antibodies
  • Description
    Mouse monoclonal [AF24G5] to ICAD
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human ICAD.
    Database link: O00273

  • Positive control
    • Hela, A431, HepG2, 293T and Jurkat cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab75514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • Function
    Inhibitor of the caspase-activated DNase (DFF40).
  • Sequence similarities
    Contains 1 CIDE-N domain.
  • Post-translational
    modifications
    Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • A330085O09Rik antibody
    • Caspase activated deoxyribonuclease inhibitor short form antibody
    • DFF 1 antibody
    • DFF 45 antibody
    • DFF alpha antibody
    • DFF-45 antibody
    • DFF1 antibody
    • DFF35 antibody
    • DFF45 antibody
    • DFFA antibody
    • Dffa DNA fragmentation factor, alpha subunit antibody
    • DFFA_HUMAN antibody
    • DNA fragmentation factor 45 kDa subunit antibody
    • DNA Fragmentation Factor Alpha Subunit antibody
    • DNA fragmentation factor subunit alpha antibody
    • DNA fragmentation factor, 45 kD, alpha subunit antibody
    • DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1 antibody
    • DNA fragmentation factor, 45kDa, alpha polypeptide antibody
    • DNA fragmentation factor, alpha subunit antibody
    • DNAation factor 45 kDa subunit antibody
    • H13 antibody
    • ICAD antibody
    • ICAD L antibody
    • ICAD S antibody
    • Inhibitor of CAD antibody
    • Inhibitor of Caspase Activated DNase antibody
    • MGC143066 antibody
    • OTTHUMP00000001903 antibody
    • OTTHUMP00000001904 antibody
    • RP23 121D17.3 antibody
    see all

Images

  • All lanes : Anti-ICAD antibody [AF24G5] (ab75514) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HepG2 cell lysate

    Predicted band size: 37 kDa

  • Immunoprecipitation analysis of HeLa cells.

    Lane 1 : HeLa Cell Lysate
    Lane 2 : Precipitated from HeLa Cell Lysate 200ug using ab75514 2ug
    Lane 3 : Precipitated from HeLa Cell Lysate 200ug using ab75514 5ug
    Lane 4 : Precipitated from HeLa using LF-MA0300 5ug


    Primary antibody usage : Rabbit polyclonal to ICAD Anti-ICAD 1/2000 for over night, 4℃.
    Secondary antibody usage : Goat Anti-Rabbit IgG 1/5000 for 1Hr, RT.

     

  • All lanes : Anti-ICAD antibody [AF24G5] (ab75514) at 1/2000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : 293T cell lysate
    Lane 5 : Jurkat cell lysate

    Predicted band size: 37 kDa
    Observed band size: 37 kDa

References

ab75514 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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