Recombinant
RabMAb

Anti-ICAD antibody [EPR4220] (ab108924)

Overview

  • Product name
    Anti-ICAD antibody [EPR4220]
    See all ICAD primary antibodies
  • Description
    Rabbit monoclonal [EPR4220] to ICAD
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) corresponding to residues in ICAD

  • Positive control
    • Jurkat cell lysate, HeLa cell lysate; human breast carcinoma tissue; HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Inhibitor of the caspase-activated DNase (DFF40).
  • Sequence similarities
    Contains 1 CIDE-N domain.
  • Post-translational
    modifications
    Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • A330085O09Rik antibody
    • Caspase activated deoxyribonuclease inhibitor short form antibody
    • DFF 1 antibody
    • DFF 45 antibody
    • DFF alpha antibody
    • DFF-45 antibody
    • DFF1 antibody
    • DFF35 antibody
    • DFF45 antibody
    • DFFA antibody
    • Dffa DNA fragmentation factor, alpha subunit antibody
    • DFFA_HUMAN antibody
    • DNA fragmentation factor 45 kDa subunit antibody
    • DNA Fragmentation Factor Alpha Subunit antibody
    • DNA fragmentation factor subunit alpha antibody
    • DNA fragmentation factor, 45 kD, alpha subunit antibody
    • DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1 antibody
    • DNA fragmentation factor, 45kDa, alpha polypeptide antibody
    • DNA fragmentation factor, alpha subunit antibody
    • DNAation factor 45 kDa subunit antibody
    • H13 antibody
    • ICAD antibody
    • ICAD L antibody
    • ICAD S antibody
    • Inhibitor of CAD antibody
    • Inhibitor of Caspase Activated DNase antibody
    • MGC143066 antibody
    • OTTHUMP00000001903 antibody
    • OTTHUMP00000001904 antibody
    • RP23 121D17.3 antibody
    see all

Images

  • Immunohistochemical staining of paraffin-embedded human breast carcinoma using ab108924 at a diltion of 1/500.
  • Immunofluorescent staining of HeLa cells using ab108924 at a dilution of 1/250.
  • All lanes : Anti-ICAD antibody [EPR4220] (ab108924) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 10 µg/ml per lane.


    Predicted band size : 37 kDa

References

ab108924 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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