Overview

  • Product nameAnti-ICAM1 antibody [15.2]
    See all ICAM1 primary antibodies
  • Description
    Mouse monoclonal [15.2] to ICAM1
  • SpecificityBlocks binding of LFA-1 & infected RBCs to ICAM1.
  • Tested applicationsSuitable for: WB, Inhibition Assay, Flow Cyt, IHC-Fr, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human monocytes.

  • Positive control
    • Tonsil

Properties

Applications

Our Abpromise guarantee covers the use of ab20 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 58 kDa.
Inhibition Assay Use at an assay dependent concentration. PubMed: 23825944
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
    • Sequence similaritiesBelongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      modifications
      Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Antigen identified by monoclonal antibody BB2 antibody
      • BB 2 antibody
      • BB2 antibody
      • CD 54 antibody
      • CD_antigen=CD54 antibody
      • CD54 antibody
      • Cell surface glycoprotein P3.58 antibody
      • Human rhinovirus receptor antibody
      • ICAM 1 antibody
      • ICAM-1 antibody
      • ICAM1 antibody
      • ICAM1_HUMAN antibody
      • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
      • Intercellular adhesion molecule 1 antibody
      • Major group rhinovirus receptor antibody
      • MALA 2 antibody
      • MALA2 antibody
      • MyD 10 antibody
      • MyD10 antibody
      • P3.58 antibody
      • Surface antigen of activated B cells, BB2 antibody
      see all

    Anti-ICAM1 antibody [15.2] images

    • All lanes : Anti-ICAM1 antibody [15.2] (ab20) at 5 µg/ml

      Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
      Lane 2 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
      Lane 3 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size : 58 kDa
      Observed band size : 58 kDa

    References for Anti-ICAM1 antibody [15.2] (ab20)

    This product has been referenced in:
    • Turner L  et al. Severe malaria is associated with parasite binding to endothelial protein C receptor. Nature 498:502-5 (2013). Read more (PubMed: 23739325) »
    • Avril M  et al. DC8 and DC13 var genes associated with severe malaria bind avidly to diverse endothelial cells. PLoS Pathog 9:e1003430 (2013). Inhibition Assay ; Human . Read more (PubMed: 23825944) »

    See all 8 Publications for this product

    Product Wall

    Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality....

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    Thank you for the response.

    Any monoclonal antibody is produced by a single immune cell, which has been cloned. These will only recognize a single epitope of the target protein. This epitope typically is 5-10 amino acids in length. Therefore...

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    While many of our ICAM1 antibodies have been tested in Western blot on human and are guaranteed to work under the terms of our Abpromise, in this instance I would recommend one of the following products. ab96485:http://www.abcam.com/ICAM1-antibody-6...

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    Balb/c is a mouse strain

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Urothelium, Bladder Cancer)
    Specification Urothelium, Bladder Cancer
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated
    Username

    Mr. Rudolf Jung

    Verified customer

    Submitted Aug 22 2006

    This antibody does not contain azide

    Yes, all abs 2952-2955 are IgG1. Abcam has a FITC conjugated sheep anti-mouse IgG secondary, product code ab11-500, which may be suitable for your needs.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"