• Product name
    Anti-ICAM1 antibody [15.2]
    See all ICAM1 primary antibodies
  • Description
    Mouse monoclonal [15.2] to ICAM1
  • Host species
  • Tested applications
    Suitable for: WB, Inhibition Assay, Flow Cyt, IHC-Fr, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human monocytes.

  • Positive control
    • WB: SK N BE, Saos-2 and HT1080 whole cell lysate. Flow Cytometry: HK-2 cells stimulated with TGF-beta1. IHC: Human amniotic membrane tissues.



Our Abpromise guarantee covers the use of ab20 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
Inhibition Assay Use at an assay dependent concentration. PubMed: 23825944
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
    • Sequence similarities
      Belongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • Antigen identified by monoclonal antibody BB2 antibody
      • BB 2 antibody
      • BB2 antibody
      • CD 54 antibody
      • CD_antigen=CD54 antibody
      • CD54 antibody
      • Cell surface glycoprotein P3.58 antibody
      • Human rhinovirus receptor antibody
      • ICAM 1 antibody
      • ICAM-1 antibody
      • ICAM1 antibody
      • ICAM1_HUMAN antibody
      • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
      • Intercellular adhesion molecule 1 antibody
      • Major group rhinovirus receptor antibody
      • MALA 2 antibody
      • MALA2 antibody
      • MyD 10 antibody
      • MyD10 antibody
      • P3.58 antibody
      • Surface antigen of activated B cells, BB2 antibody
      see all


    • Expression of proteins with functions involved in ocular disease and wound healing in AM substrates.

      Proteins detected include growth factors and biomarkers (A) cell adhesion, cytokine and angiogenesis markers (B) metalloproteases and (C) neurotrophic factors (D). The collective pattern in staining demonstrates comparable levels of expression in trehalose and raffinose treated AM compared to fresh and increased expression compared to cryopreserved AM. Positive staining is represented as green or yellow and AEC nuclei were counterstained with DAPI (blue). Images shown are representative of triplicate experiments carried out on three donor membranes. Scale bar, 100 µm.

    • ICAM-1 expression on HK-2 cells.

      HK-2 cells (Mock) and HK-2 cells stimulated with TGF-β1 (10.0, 1.0 and 0.1 ng/ml) were stained with ab20 and isotype control mAb (A). HK-2 cells (Mock) and HK-2 cells stimulated with TGF-β1 (10.0 ng/ml) were stained with ab20 and isotype control mAb at 24 hrs, 48 hrs and 72 hrs after TGF-β1 stimulation (B). Cells were analyzed by flow cytometry. Closed histogram: ICAM-1-stained cells. Dotted line: isotype control stained cells. MFI: mean fluorescence intensity.

    • All lanes : Anti-ICAM1 antibody [15.2] (ab20) at 5 µg/ml

      Lane 1 : SK N BE (Human neuroblastoma) whole cell lysate
      Lane 2 : Saos-2 (Human epithelial-like osteosarcoma cell line) whole cell lysate
      Lane 3 : HT1080 (Human fibrosarcoma) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size: 58 kDa
      Observed band size: 58 kDa

    • Binding levels of P. falciparum-infected erythrocytes increase after endothelial cell selection.


    This product has been referenced in:
    • Yasen A  et al. Release of HIV-1 sequestered in the vesicles of oral and genital mucosal epithelial cells by epithelial-lymphocyte interaction. PLoS Pathog 13:e1006247 (2017). Read more (PubMed: 28241053) »
    • Avril M  et al. Interaction between Endothelial Protein C Receptor and Intercellular Adhesion Molecule 1 to Mediate Binding of Plasmodium falciparum-Infected Erythrocytes to Endothelial Cells. MBio 7:N/A (2016). Inhibition Assay . Read more (PubMed: 27406562) »

    See all 12 Publications for this product

    Customer reviews and Q&As

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    Abcam has not validated the combination of species/application used in this Abreview.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Human Tissue sections (Urothelium, Bladder Cancer)
    Urothelium, Bladder Cancer
    Antigen retrieval step
    Heat mediated

    Mr. Rudolf Jung

    Verified customer

    Submitted Aug 22 2006


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