Overview

  • Product name
    Anti-ICAM1 antibody [1A29]
    See all ICAM1 primary antibodies
  • Description
    Mouse monoclonal [1A29] to ICAM1
  • Host species
    Mouse
  • Tested applications
    Suitable for: Blocking, WB, IP, ICC/IF, IHC-P, Neutralising, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Full length protein corresponding to Rat ICAM1. Rat lymph node stroma
    Database link: Q00238

  • Positive control
    • Mouse kidney, lung and spleen tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab171123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 57 kDa.
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 2 µg/ml.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Neutralising Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen identified by monoclonal antibody BB2 antibody
    • BB 2 antibody
    • BB2 antibody
    • CD 54 antibody
    • CD_antigen=CD54 antibody
    • CD54 antibody
    • Cell surface glycoprotein P3.58 antibody
    • Human rhinovirus receptor antibody
    • ICAM 1 antibody
    • ICAM-1 antibody
    • ICAM1 antibody
    • ICAM1_HUMAN antibody
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
    • Intercellular adhesion molecule 1 antibody
    • Major group rhinovirus receptor antibody
    • MALA 2 antibody
    • MALA2 antibody
    • MyD 10 antibody
    • MyD10 antibody
    • P3.58 antibody
    • Surface antigen of activated B cells, BB2 antibody
    see all

Images

  • Immunohistochemical analysis of deparaffinized mouse kidney tissue, labeling ICAM1 with ab171123 at 1/100 dilution (left image) compared with a negative control without primary antibody (right image). Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin.

  • Flow cytometry analysis of ICAM1 in C6 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a ICAM1 monoclonal antibody (ab171123) at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

  • Immunocytochemical analysis of A439 cells staining ICAM1 with ab171123 at 1ug/ml.

    Cells were fixed with 4% paraformaldehyde for 15 minutes, permeablilized with 0.25% Trition X-100 for 10 minutes and blocked with 5% BSA for 1 hour at room temparature. Cells were incubated with primary antibody at room temperature for 3 hours. Goat Anti-Mouse IgG (H&L) (Alexa Fluor® 488) was used as a secondary antibody.

  • Flow cytometry analysis of ICAM1 in Raji cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a ICAM1 monoclonal antibody (ab171123) at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

  • Flow cytometry analysis of ICAM1 in Ramos cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a ICAM1 monoclonal antibody (ab171123) at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

  • Immunohistochemical analysis of deparaffinized mouse spleen tissue, labeling ICAM1 with ab171123 at 1/100 dilution (left image) compared with a negative control without primary antibody (right image). Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin.

  • Immunohistochemical analysis of deparaffinized mouse lung tissue, labeling ICAM1 with ab171123 at 1/100 dilution (left image) compared with a negative control without primary antibody (right image). Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin.

References

This product has been referenced in:
  • Guo J  et al. Interleukin-1ß induces intercellular adhesion molecule-1 expression, thus enhancing the adhesion between mesenchymal stem cells and endothelial progenitor cells via the p38 MAPK signaling pathway. Int J Mol Med 41:1976-1982 (2018). Read more (PubMed: 29393395) »
  • Gu R  et al. Glucocorticoid-Induced Leucine Zipper Suppresses ICAM-1 and MCP-1 Expression by Dephosphorylation of NF-?B p65 in Retinal Endothelial Cells. Invest Ophthalmol Vis Sci 58:631-641 (2017). Read more (PubMed: 28129426) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for your interest in the ICAM-1 monoclonal antibody ab171123. The reference I found that describes neutralization/blocking of ICAM-1 with the antibody used the antibody in an in vivo study of the effect of the antibody (and others) on leukoc...

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