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Tissue/ cell preparation (Rat) -Ax cells (a HEV derived cell line).
Our Abpromise guarantee covers the use of ab33894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.|
|Functional Studies||Use at an assay dependent concentration. ab33894 functionally inhibits homotypic aggregation of PHA blasts.|
|Flow Cyt||1/25 - 1/50. Use 10ul of the suggested working dilution to label 106 cells in 100ul.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.