Overview

  • Product name
    Anti-ICAM1 antibody [1A29] (FITC)
    See all ICAM1 primary antibodies
  • Description
    Mouse monoclonal [1A29] to ICAM1 (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat Ax cell line (HEV derived cells).

Properties

Applications

Our Abpromise guarantee covers the use of ab23835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: 1/25 - 1/100. Use 10µl working dilution for 106 cells in 100µl.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
    • Sequence similarities
      Belongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      modifications
      Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Antigen identified by monoclonal antibody BB2 antibody
      • BB 2 antibody
      • BB2 antibody
      • CD 54 antibody
      • CD_antigen=CD54 antibody
      • CD54 antibody
      • Cell surface glycoprotein P3.58 antibody
      • Human rhinovirus receptor antibody
      • ICAM 1 antibody
      • ICAM-1 antibody
      • ICAM1 antibody
      • ICAM1_HUMAN antibody
      • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
      • Intercellular adhesion molecule 1 antibody
      • Major group rhinovirus receptor antibody
      • MALA 2 antibody
      • MALA2 antibody
      • MyD 10 antibody
      • MyD10 antibody
      • P3.58 antibody
      • Surface antigen of activated B cells, BB2 antibody
      see all

    Anti-ICAM1 antibody [1A29] (FITC) images

    • ab23875 staining ICAM1 in stimulated rat spleen cells by Flow cytometry.

    References for Anti-ICAM1 antibody [1A29] (FITC) (ab23835)

    This product has been referenced in:
    • Tamatani T  et al. Molecular mechanisms underlying lymphocyte recirculation. II. Differential regulation of LFA-1 in the interaction between lymphocytes and high endothelial cells. Eur J Immunol 21:855-8 (1991). Read more (PubMed: 1672652) »
    • Tamatani T & Miyasaka M Identification of monoclonal antibodies reactive with the rat homolog of ICAM-1, and evidence for a differential involvement of ICAM-1 in the adherence of resting versus activated lymphocytes to high endothelial cells. Int Immunol 2:165-71 (1990). Read more (PubMed: 2088483) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Rat Cell (Rat primary endothelial cells)
    Permeabilization
    No
    Gating Strategy
    FITC positive cells, gated based on a negative control
    Specification
    Rat primary endothelial cells
    Preparation
    Cell harvesting/tissue preparation method: Primary rat cells were stained with ab23835 immediately after isolation and run on FACS, around 13% of the cells were positive, which were also what was expected (the remaining cells where fibroblasts, myocytes etc.)
    Sample buffer: The cells where stained in 5% FBS-PBS and sorted into 10% FBS-PBS
    Fixation
    No fixation
    Username

    Abcam user community

    Verified customer

    Submitted Jul 10 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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