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Our Abpromise guarantee covers the use of ab67217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 1 µg/ml. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 90 kDa.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Human tonsil tissue sections could be used as positive control. 5 minutes heating in microwave in Na Citrate buffer pH 6.0 is recommended for antigen retrieval.|
|IHC-Fr||Use a concentration of 5 - 10 µg/ml.|
|ICC||Use at an assay dependent concentration.|