Overview

  • Product nameAnti-ICAM1 antibody [6.5B5]
    See all ICAM1 primary antibodies
  • Description
    Mouse monoclonal [6.5B5] to ICAM1
  • Specificityab33090 reacts with activated endothelial cells, epithelial cells, lymphocytes and monocytes. Clone 6.5B5 blocks LFA-1 mediated adhesion to ICAM-1.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, ELISA, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human ICAM1, domain 1

Properties

Applications

Our Abpromise guarantee covers the use of ab33090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IHC-Fr
ELISA
IP
  • Application notesELISA: Use at an assay dependent dilution. Dectection only.
    Flow Cyt: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.


    Is unsuitable for WB.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
    • Sequence similaritiesBelongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      modifications
      Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Antigen identified by monoclonal antibody BB2 antibody
      • BB 2 antibody
      • BB2 antibody
      • CD 54 antibody
      • CD_antigen=CD54 antibody
      • CD54 antibody
      • Cell surface glycoprotein P3.58 antibody
      • Human rhinovirus receptor antibody
      • ICAM 1 antibody
      • ICAM-1 antibody
      • ICAM1 antibody
      • ICAM1_HUMAN antibody
      • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
      • Intercellular adhesion molecule 1 antibody
      • Major group rhinovirus receptor antibody
      • MALA 2 antibody
      • MALA2 antibody
      • MyD 10 antibody
      • MyD10 antibody
      • P3.58 antibody
      • Surface antigen of activated B cells, BB2 antibody
      see all

    References for Anti-ICAM1 antibody [6.5B5] (ab33090)

    This product has been referenced in:
    • Burton VJ  et al. Bone morphogenetic protein receptor II regulates pulmonary artery endothelial cell barrier function. Blood 117:333-41 (2011). ELISA ; Human . Read more (PubMed: 20724539) »
    • Montefort S  et al. Intercellular adhesion molecule-1 (ICAM-1) and endothelial leucocyte adhesion molecule-1 (ELAM-1) expression in the bronchial mucosa of normal and asthmatic subjects. Eur Respir J 5:815-23 (1992). Read more (PubMed: 1379941) »

    See all 3 Publications for this product

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    Since this antibody is a tissue culture supernatant, it is not purified and the exact IgG concentration has not been determined. Tissue culture supernatants generally have total protein concentrations around 1 - 3...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"