Overview

  • Product nameAnti-ICAM1 antibody
    See all ICAM1 primary antibodies
  • Description
    Rabbit polyclonal to ICAM1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chimpanzee, Chinese Hamster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse ICAM1.

  • Positive control
    • This antibody gave a positive signal in Western Blot within the following whole cell lysates: Raw264.7; C2C12; K562; HUVEC. This antibody also gave a positive signal in Immunohistochemistry within Human normal Lung formalin fixed paraffin embedded tissue section.

Properties

Applications

Our Abpromise guarantee covers the use of ab124759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 58 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • FunctionICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen identified by monoclonal antibody BB2 antibody
    • BB 2 antibody
    • BB2 antibody
    • CD 54 antibody
    • CD_antigen=CD54 antibody
    • CD54 antibody
    • Cell surface glycoprotein P3.58 antibody
    • Human rhinovirus receptor antibody
    • ICAM 1 antibody
    • ICAM-1 antibody
    • ICAM1 antibody
    • ICAM1_HUMAN antibody
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
    • Intercellular adhesion molecule 1 antibody
    • Major group rhinovirus receptor antibody
    • MALA 2 antibody
    • MALA2 antibody
    • MyD 10 antibody
    • MyD10 antibody
    • P3.58 antibody
    • Surface antigen of activated B cells, BB2 antibody
    see all

Anti-ICAM1 antibody images

  • All lanes : Anti-ICAM1 antibody (ab124759) at 1 µg/ml

    Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
    Lane 2 : C2C12 (Mouse myoblast cell line) Whole Cell Lysate
    Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 4 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 99 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,70 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

    The predicted molecular weight of ICAM1 is 58 kDa (SwissProt), however we expect to observe a banding pattern around 100 kDa. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124759 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • IHC image of ICAM1 staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (epitope retrieval solution 2) for 20 mins. The section was then incubated with ab124759, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-ICAM1 antibody (ab124759)

This product has been referenced in:
  • Shi KL  et al. Atorvastatin antagonizes the visfatin-induced expression of inflammatory mediators via the upregulation of NF-?B activation in HCAECs. Oncol Lett 12:1438-1444 (2016). Human . Read more (PubMed: 27446449) »
  • Ahn J  et al. Intrinsic self-DNA triggers inflammatory disease dependent on STING. J Immunol 193:4634-42 (2014). Read more (PubMed: 25261479) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"