Synthetic peptide corresponding to Rat ICAM1 aa 400-500 conjugated to keyhole limpet haemocyanin. Database link: Q00238 (Peptide available as ab167147)
This antibody also gave a positive signal in NIH3T3 whole cell lysate as well as the following tissue lysates: Rat Heart; Rat Thymus; Mouse Heart; Mouse Thymus. In IHC-P, it gave a positive results in FFPE Rat Lung tissue sections.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Belongs to the immunoglobulin superfamily. ICAM family. Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Antigen identified by monoclonal antibody BB2 antibody
BB 2 antibody
CD 54 antibody
Cell surface glycoprotein P3.58 antibody
Human rhinovirus receptor antibody
ICAM 1 antibody
intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
Intercellular adhesion molecule 1 antibody
Major group rhinovirus receptor antibody
MALA 2 antibody
MyD 10 antibody
Surface antigen of activated B cells, BB2 antibody
Western blot - Anti-ICAM1 antibody (ab124760)
All lanes : Anti-ICAM1 antibody (ab124760) at 1 µg/ml
Lane 1 : Heart (Rat) Tissue Lysate Lane 2 : Thymus (Rat) Tissue Lysate Lane 3 : Heart (Mouse) Tissue Lysate Lane 4 : Thymus (Mouse) Tissue Lysate Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 60 kDa Observed band size : 60 kDa Additional bands at : 35 kDa,45 kDa,85 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124760 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody (ab124760)This image is courtesy of an anonymous Abreview
ab124760 staining ICAM1 in mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in EDTA-buffer, pH 9.0. Samples were incubated with primary antibody (1/100 in PBS) for 20 hours at 20°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
IHC image of ab124760 staining in Rat Lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab124760, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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