Overview

  • Product nameAnti-ICAM1 antibody (Biotin)
    See all ICAM1 primary antibodies
  • Description
    Rabbit polyclonal to ICAM1 (Biotin)
  • ConjugationBiotin
  • SpecificityThe antibody reacts with both intact human ICAM-1, CD54 and with soluble human ICAM-1.
  • Tested applicationsSandwich ELISA, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Chimpanzee, Baboon, Cynomolgus Monkey, Rhesus monkey
  • Immunogen

    Full length protein (Human).

  • Positive control
    • Fresh skin biopsy (small fresh skin biopsy incubated for 4 hours with TNF (1 ng/ml), IL-1 (100 u/ml) or LPS (1 ìg/ml) in tissue culture medium at 37oC).

Properties

Applications

Our Abpromise guarantee covers the use of ab7815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [MEM-111] to ICAM1 (ab2213). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab2213 as Capture.
IHC-Fr Use at an assay dependent concentration. Fix with acetone.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Target

  • FunctionICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen identified by monoclonal antibody BB2 antibody
    • BB 2 antibody
    • BB2 antibody
    • CD 54 antibody
    • CD_antigen=CD54 antibody
    • CD54 antibody
    • CD54 antigen antibody
    • Cell surface glycoprotein P3.58 antibody
    • Human rhinovirus receptor antibody
    • ICAM 1 antibody
    • ICAM-1 antibody
    • ICAM1 antibody
    • ICAM1_HUMAN antibody
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
    • Intercellular adhesion molecule 1 antibody
    • Major group rhinovirus receptor antibody
    • MALA 2 antibody
    • MALA2 antibody
    • MyD 10 antibody
    • MyD10 antibody
    • P3.58 antibody
    • Surface antigen of activated B cells antibody
    • Surface antigen of activated B cells, BB2 antibody
    see all

Anti-ICAM1 antibody (Biotin) images

References for Anti-ICAM1 antibody (Biotin) (ab7815)

This product has been referenced in:
  • Rohlena J  et al. Endothelial CD81 is a marker of early human atherosclerotic plaques and facilitates monocyte adhesion. Cardiovasc Res 81:187-96 (2009). Electron Microscopy ; Human . Read more (PubMed: 18805782) »
  • Fransson J  et al. Rapid induction of apoptosis in B-cell lymphoma by functionally isolated human antibodies. Int J Cancer 119:349-58 (2006). Blocking ; Human . Read more (PubMed: 16477633) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Nov 27 2013

Apres plusieurs conversations avec notre service commercial j’ai réussi d’ajouter l’anticorps ab7815 non conjugué au catalogue. La référence est ab170497.

Thank you for the response.

Any monoclonal antibody is produced by a single immune cell, which has been cloned. These will only recognize a single epitope of the target protein. This epitope typically is 5-10 amino acids in length. Therefore...

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Thank you for contacting us.

ab7815 and ab33451 have been tested with Frozen sections only and we do not have any empirical data to support their performance with paraffin tissues.

I have found the following ICAM1 and Monocyte + mac...

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Thank you for your email.

I have a couple recommendation for your customer when using IHC-Fr.

1.) I strongly suggest to run a positive control. ICAM1 belongs to the homing receptors and to my knowledge should not be expressed in bra...

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Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody. I can confirm that ab7815 is covered by our Abpromise g...

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I can confirm that we didn't test the antibody (ab106777: Anti-VCAM1) in Rabbit tissue, as soon as we have further information we will update our on-line datasheet accordingly.

Thank you for your interest.



The reactivity of this antibody (ab2213) with rabbit samples on Western blot application is probable but it has not been tested yet.



Hope this will be useful for you!

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 40 µg
Specification brain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Alicia Moreno

Verified customer

Submitted Sep 06 2010

Thank you for contacting us for technical support with ab7815. I'm sorry to hear your recent vial of antibody has not given you successful staining. I recall you contacting us last summer about this antibody, having good staining in paraffin sections i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"