• Product nameAnti-ICAM1 antibody [EP1442Y]
    See all ICAM1 primary antibodies
  • Description
    Rabbit monoclonal [EP1442Y] to ICAM1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ICAM1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control
    • Raji cell membrane lysate Human tonsil tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:
    Anti-ICAM1 antibody (HRP) [EP1442Y] (ab195528)

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.



Our Abpromise guarantee covers the use of ab53013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 89 kDa (predicted molecular weight: 58 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
    • Sequence similaritiesBelongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Antigen identified by monoclonal antibody BB2 antibody
      • BB 2 antibody
      • BB2 antibody
      • CD 54 antibody
      • CD_antigen=CD54 antibody
      • CD54 antibody
      • Cell surface glycoprotein P3.58 antibody
      • Human rhinovirus receptor antibody
      • ICAM 1 antibody
      • ICAM-1 antibody
      • ICAM1 antibody
      • ICAM1_HUMAN antibody
      • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
      • Intercellular adhesion molecule 1 antibody
      • Major group rhinovirus receptor antibody
      • MALA 2 antibody
      • MALA2 antibody
      • MyD 10 antibody
      • MyD10 antibody
      • P3.58 antibody
      • Surface antigen of activated B cells, BB2 antibody
      see all

    Anti-ICAM1 antibody [EP1442Y] images

    • ab53013, at a 1/50 dilution, staining Human ICAM1 in Tonsil using Immunohistochemistry, Paraffin embedded tissue.
    • Overlay histogram showing Raji cells stained with ab53013 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53013, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
    • Anti-ICAM1 antibody [EP1442Y] (ab53013) at 1/2000 dilution + Raji cell lysate at 10 µg

      goat anti-rabbit HRP labelled at 1/2000 dilution

      Predicted band size : 58 kDa
      Observed band size : 89 kDa (why is the actual band size different from the predicted?)

    References for Anti-ICAM1 antibody [EP1442Y] (ab53013)

    This product has been referenced in:
    • Shi H  et al. Long non-coding RNA expression profile in minor salivary gland of primary Sjögren's syndrome. Arthritis Res Ther 18:109 (2016). IHC ; Human . Read more (PubMed: 27188286) »
    • Sumbria RK  et al. A murine model of inflammation-induced cerebral microbleeds. J Neuroinflammation 13:218 (2016). IHC ; Mouse . Read more (PubMed: 27577728) »

    See all 11 Publications for this product

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