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Tissue/ cell preparation (Human). (Burkitt's lymphoma cell line RAJI).
Our Abpromise guarantee covers the use of ab2213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 25 µg/ml. Use at a dilution of 1/1000 for Indirect ELISA, 1hr, RT. The antibody reacts with tissue - endothelial cell line derived from bovine pulmonary artery.|
|WB||Use at an assay dependent concentration. Use under non-reducing conditions.|
|IHC-P||Use a concentration of 10 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use a concentration of 2 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|Sandwich ELISA||Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to ICAM1 (Biotin) (ab7815). For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab7815 as Detection.|
Lower bands represent tubulin as a loading control.
ab2213 staining human PBL cells by flow cytometry. Cells were formaldehyde fixed and gated on B-cells. The primary antibody was incubated with the sample for 30 minutes at 25°C. The secondary antibody was ab6810 (a FITC conjugated chicken polyclonal to mouse IgG - H&L). Both antibodies were used undiluted.
This image is courtesy of an anonymous Abreview