Overview

  • Product name
    Anti-ICAM1 antibody [YN1/1.7.4]
    See all ICAM1 primary antibodies
  • Description
    Rat monoclonal [YN1/1.7.4] to ICAM1
  • Tested applications
    Suitable for: WB, IP, IHC-Fr, Functional Studies, ICC, Flow Cyt, Blocking, IHC-P, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Mouse ICAM1.

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Mouse lung.

Properties

Applications

Our Abpromise guarantee covers the use of ab119871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. Application note:acetone fixation
Functional Studies Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use a concentration of 1 µg/ml.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Blocking Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-FoFr 1/200.

Target

  • Function
    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen identified by monoclonal antibody BB2 antibody
    • BB 2 antibody
    • BB2 antibody
    • CD 54 antibody
    • CD_antigen=CD54 antibody
    • CD54 antibody
    • Cell surface glycoprotein P3.58 antibody
    • Human rhinovirus receptor antibody
    • ICAM 1 antibody
    • ICAM-1 antibody
    • ICAM1 antibody
    • ICAM1_HUMAN antibody
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
    • Intercellular adhesion molecule 1 antibody
    • Major group rhinovirus receptor antibody
    • MALA 2 antibody
    • MALA2 antibody
    • MyD 10 antibody
    • MyD10 antibody
    • P3.58 antibody
    • Surface antigen of activated B cells, BB2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse atheroslerotic lesions of aortic root tissue sections labeling ICAM1 with ab119871 at 1/200 dilution. The aortic root was rapidly fixed and harvested at 4°C in phosphate-buffered 4% paraformaldehyde, pH 7.0–7.4, then embedded in paraffin wax sectioned by semi-automated rotary microtome.

  • ab119871 staining ICAM1 in mouse olfactory epithelium tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with 1% paraformaldehyde + lysine + periodate (1% PLP) and permeablized and blocked with 10% donkey serum + 5% nonfat dry milk + 4% BSA + 1% triton X-100. The sample was incubated with primary antibody (1/200) for 1 hour at 27°C. An Alexa Fluor® 488-conjugated donkey anti-rat polyclonal (1/150) was used as the secondary antibody.

    See Abreview

  • IHC image of ICAM1 staining in Mouse normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with EDTA based pH 9.0 solution, (epitope retrieval solution 2) for 20 mins. The section was then incubated with ab119871, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Flow Cytometry analysis of mouse thymocytes labeling ICAM1 with Anti-ICAM1 antibody [YN1/1.7.4] (ab119871).

References

This product has been referenced in:
  • Sun H  et al. Attenuation of atherosclerotic lesions in diabetic apolipoprotein E-deficient mice using gene silencing of macrophage migration inhibitory factor. J Cell Mol Med 19:836-49 (2015). IHC-P ; Mouse . Read more (PubMed: 25661015) »
  • Weber EW  et al. TRPC6 is the endothelial calcium channel that regulates leukocyte transendothelial migration during the inflammatory response. J Exp Med 212:1883-99 (2015). Read more (PubMed: 26392222) »

See all 7 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step
None
Sample
Mouse Tissue sections (Olfactory epithelium)
Specification
Olfactory epithelium
Permeabilization
Yes - .1% TritonX-100 in block
Fixative
1% Paraformaldehyde+lysine+periodate (1%PLP)
Username

Dr. Schwob Lab

Verified customer

Submitted Jun 10 2014

Thank you for contacting us.

The predicted size, based on the amino acid sequence before any post-translation modifications, is 58 kDa. I do not have the western blot data for this particular antibody, but 58 kDa is the size of the proteins ...

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Thanks you for your email.
In my previous email there is a correction about the expiry date. The expiry date should be read as 25-08-2012.
I have asked lab to release the immunogen information of these antibodies; I will be able to send the a...

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