The details of the immunogen for this antibody are not available.
Our Abpromise guarantee covers the use of ab25375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent dilution. PubMed: 2573511Use at an assay dependent dilution (see PubMed 2573511).|
|IHC-Fr||Use at an assay dependent dilution. PubMed: 8943409Use at an assay dependent dilution (see PubMed 8943409).|
|Blocking||Use at an assay dependent dilution. PubMed: 2981916Use at an assay dependent dilution (see PubMed 2981916).|
|WB||Use at an assay dependent dilution. Detects a band of approximately 95 kDa (predicted molecular weight: 85-110 kDa). PubMed: 8095510|
Flow Cytometry analysis of BALB/c mouse splenocytes labeling ICAM1 with ab25375 at 0.3 μg/106 cells (purple). A Mouse Anti-Rat IgG2b-CY5 was used as the secondary antibody. Grey - Isotype Control, Rat IgG2b-UNLB, followed by Mouse Anti-Rat IgG2b-CY5.
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