Overview

  • Product nameAnti-ICAM1 antibody [YN1/1.7.4]
    See all ICAM1 primary antibodies
  • Description
    Rat monoclonal [YN1/1.7.4] to ICAM1
  • Tested applicationsSuitable for: IP, IHC-Fr, Blocking, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • PMA or LPS stimulated T-cell line such as EL-4 or 18-81

Properties

Applications

Our Abpromise guarantee covers the use of ab25375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution. PubMed: 2573511Use at an assay dependent dilution (see PubMed 2573511).
IHC-Fr Use at an assay dependent dilution. PubMed: 8943409Use at an assay dependent dilution (see PubMed 8943409).
Blocking Use at an assay dependent dilution. PubMed: 2981916Use at an assay dependent dilution (see PubMed 2981916).
WB Use at an assay dependent dilution. Detects a band of approximately 95 kDa (predicted molecular weight: 85-110 kDa). PubMed: 8095510

Target

  • FunctionICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen identified by monoclonal antibody BB2 antibody
    • BB 2 antibody
    • BB2 antibody
    • CD 54 antibody
    • CD_antigen=CD54 antibody
    • CD54 antibody
    • Cell surface glycoprotein P3.58 antibody
    • Human rhinovirus receptor antibody
    • ICAM 1 antibody
    • ICAM-1 antibody
    • ICAM1 antibody
    • ICAM1_HUMAN antibody
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
    • Intercellular adhesion molecule 1 antibody
    • Major group rhinovirus receptor antibody
    • MALA 2 antibody
    • MALA2 antibody
    • MyD 10 antibody
    • MyD10 antibody
    • P3.58 antibody
    • Surface antigen of activated B cells, BB2 antibody
    see all

Anti-ICAM1 antibody [YN1/1.7.4] images

  • Flow Cytometry analysis of BALB/c mouse splenocytes labeling ICAM1 with ab25375 at 0.3 μg/106 cells (purple). A Mouse Anti-Rat IgG2b-CY5 was used as the secondary antibody. Grey - Isotype Control, Rat IgG2b-UNLB, followed by Mouse Anti-Rat IgG2b-CY5.

References for Anti-ICAM1 antibody [YN1/1.7.4] (ab25375)

This product has been referenced in:
  • Pannier D  et al. High expression levels of egfl7 correlate with low endothelial cell activation in peritumoral vessels of human breast cancer. Oncol Lett 12:1422-1428 (2016). IHC-P ; Mouse . Read more (PubMed: 27446447) »
  • Mondini M  et al. Synergy of Radiotherapy and a Cancer Vaccine for the Treatment of HPV-Associated Head and Neck Cancer. Mol Cancer Ther 14:1336-45 (2015). IHC-Fr ; Mouse . Read more (PubMed: 25833837) »

See all 14 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Pulmonary Microvascular Endothelial Cells)
Permeabilization Yes - 0.5% Triton in PBS 10 min on ice
Specification Pulmonary Microvascular Endothelial Cells
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative Methanol:Acetone
Username

Jose Vazquez-Medina

Verified customer

Submitted Nov 15 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.1% Triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 13 2015

Thank you for your call today and for your interest in these antibodies.

Since we do not have a CD54 antibody that hasalready been tested in IHC-P with mouse samples, I can offer a discount off a future purchase if you'd liketest a CD54 antib...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for the response.

Any monoclonal antibody is produced by a single immune cell, which has been cloned. These will only recognize a single epitope of the target protein. This epitope typically is 5-10 amino acids in length. Therefore...

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Perdona por la tardanza en contestar, estaba chequeando con mis compañeros del laboratorio las distintas opciones de reactividad cruzada de este anticuerpo con conejo. Desgraciadamente no hay muchos datos al respecto, ya que ninguno de ellos se ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Dr. Thomas Weissensteiner

Verified customer

Submitted Oct 22 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative PLP
Permeabilization Yes - 0.05% Tween-20 washes
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 19 2009

Thank you for your enquiry. I have enquired at the originator of the product but unfortunately, they do not have the information that you are requesting. I am sorry I can't be of more assistance in this occasion. If there is anything else I can...

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Thank you for providing these details regarding your protocol. Ab25375 should detect a band at approximately 58 kDa; did you see a band at this size? This particular antibody has only so far been tested for cross-reactivity with mouse and has not yet b...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"