Anti-ICAM1 antibody [YN1/1.7.4] (Phycoerythrin) (ab25553)

Overview

  • Product nameAnti-ICAM1 antibody [YN1/1.7.4] (Phycoerythrin)
    See all ICAM1 primary antibodies
  • Description
    Rat monoclonal [YN1/1.7.4] to ICAM1 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: Flow Cyt, WB, IP, Blocking, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

Applications

Our Abpromise guarantee covers the use of ab25553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells.
WB Use at an assay dependent dilution. Detects a band of approximately 95 kDa (predicted molecular weight: 85-110 kDa).
IP Use at an assay dependent dilution.
Blocking Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

Target

  • FunctionICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen identified by monoclonal antibody BB2 antibody
    • BB 2 antibody
    • BB2 antibody
    • CD 54 antibody
    • CD_antigen=CD54 antibody
    • CD54 antibody
    • Cell surface glycoprotein P3.58 antibody
    • Human rhinovirus receptor antibody
    • ICAM 1 antibody
    • ICAM-1 antibody
    • ICAM1 antibody
    • ICAM1_HUMAN antibody
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
    • Intercellular adhesion molecule 1 antibody
    • Major group rhinovirus receptor antibody
    • MALA 2 antibody
    • MALA2 antibody
    • MyD 10 antibody
    • MyD10 antibody
    • P3.58 antibody
    • Surface antigen of activated B cells, BB2 antibody
    see all

References for Anti-ICAM1 antibody [YN1/1.7.4] (Phycoerythrin) (ab25553)

This product has been referenced in:
  • Qin L  et al. The roles of CD11/CD18 and ICAM-1 in acute Pseudomonas aeruginosa-induced pneumonia in mice. J Immunol 157:5016-21 (1996). Read more (PubMed: 8943409) »
  • Welder CA  et al. Inhibition of cell adhesion by microspheres coated with recombinant soluble intercellular adhesion molecule-1. J Immunol 150:2203-10 (1993). Read more (PubMed: 8095510) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (lymph node)
Specification lymph node
Preparation Cell harvesting/tissue preparation method: digestion collagenase
Sample buffer: PBS/BSA 1%
Permeabilization No
Gating Strategy PI negative
Username

Dr. alexandre garin

Verified customer

Submitted Jul 29 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"