Overview

  • Product nameICAM1 (CD54) Monkey ELISA Kit
    See all ICAM1 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 5%
    Overall < 5%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    1.1 U/ml
  • Range
    6.3 U/ml - 100 U/ml
  • Recovery

    95 %

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Monkey
  • Product overview

    Abcam’s ICAM1 (CD54) Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of monkey ICAM1 (CD54) concentrations in cell culture supernatant and serum (baboon, rhesus macaque).

    ICAM1 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated ICAM1 detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount ICAM1 captured on the plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.
  • Cellular localizationMembrane
  • Alternative names
    • BB2
    • CD 54
    • CD54
    • CD54 antigen
    • Cell surface glycoprotein P3.58
    • Human rhinovirus receptor
    • ICAM 1
    • Intercellular adhesion molecule 1
    • Major group rhinovirus receptor
    • MALA2
    • MGC6195
    • MyD10
    • P3.58
    • Surface antigen of activated B cells
    see all

Applications

Our Abpromise guarantee covers the use of ab119527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

ICAM1 (CD54) Monkey ELISA Kit images

  • Representative Standard Curve using ab119527.

Protocols

References for ICAM1 (CD54) Monkey ELISA Kit (ab119527)

ab119527 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"