Overview

  • Product nameICAM1 (CD54) Mouse ELISA KitSee all ICAM1 kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    24.69 pg/ml - 6000 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 93.54 84% - 103%
    Serum 94.56 84% - 103%
    Plasma 115.2 97% - 127%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s ICAM1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse ICAM1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse ICAM1 coated on a 96- well plate. Standards and samples are pipetted into the wells and ICAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse ICAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.
  • Cellular localizationMembrane
  • Alternative names
    • BB2
    • CD 54
    • CD54
    • CD54 antigen
    • Cell surface glycoprotein P3.58
    • Human rhinovirus receptor
    • ICAM 1
    • Intercellular adhesion molecule 1
    • Major group rhinovirus receptor
    • MALA2
    • MGC6195
    • MyD10
    • P3.58
    • Surface antigen of activated B cells
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

ICAM1 (CD54) Mouse ELISA Kit images

  • Representative Standard Curve

Protocols

References for ICAM1 (CD54) Mouse ELISA Kit (ab100688)

ab100688 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Althought this particular kit has not, to our knowledge, been tested with tissue homogenates, it should be compatible.

We recommend diluting the samples at least 5-fold with Assay Diluent D to minimize any effect...

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Thank you for contacting us.

The following ELISA kits for mouse ICAM, VCAM, IL-6 and TNF-alpha are suitable for mouse samples:

ab100688 (http://www.abcam.com/ICAM1-Mouse-ELISA-Kit-1-x-96-Well-Plate-ab100688.html)
ab100713 (htt...

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Thank you for your call earlier this week and for your patience while I have looked into your enquiry. I contacted the lab to ask about cross-reactivity of our mouse ICAM1 and VCAM ELISA kits with the other CAM's, and unfortunately the antibodie...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"