Mouse ICAM1 ELISA Kit (CD54) (ab100688)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 24.69 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse ICAM1 ELISA Kit (CD54)
See all ICAM1 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 20 pg/ml -
Range
24.69 pg/ml - 6000 pg/ml -
Recovery
97 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 93.54 84% - 103% Serum 94.56 84% - 103% Plasma 115.2 97% - 127% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s ICAM1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse ICAM1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse ICAM1 coated on a 96- well plate. Standards and samples are pipetted into the wells and ICAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse ICAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated anti-Mouse ICAM1 2 vials ICAM1 Microplate (12 x 8 wells) 1 unit Recombinant Mouse ICAM1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria. -
Cellular localization
Membrane -
Alternative names
- BB2
- CD 54
- CD54
see all -
Database links
- Entrez Gene: 15894 Mouse
- SwissProt: P13597 Mouse
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab100688 has been referenced in 13 publications.
- Wang WX et al. Sevoflurane preconditioning prevents acute renal injury caused by ischemia-reperfusion in mice via activation of the Nrf2 signaling pathway. Exp Ther Med 23:303 (2022). PubMed: 35340877
- Geng X et al. Interaction of RARRES1 with ICAM1 modulates macrophages to suppress the progression of kidney renal clear cell carcinoma. Front Immunol 13:982045 (2022). PubMed: 36353618
- Nam JK et al. An antibody against L1 cell adhesion molecule inhibits cardiotoxicity by regulating persistent DNA damage. Nat Commun 12:3279 (2021). PubMed: 34078883
- He X et al. Endogenous α7 nAChR Agonist SLURP1 Facilitates Escherichia coli K1 Crossing the Blood-Brain Barrier. Front Immunol 12:745854 (2021). PubMed: 34721415
- Sapoznikov A et al. Intramuscular Exposure to a Lethal Dose of Ricin Toxin Leads to Endothelial Glycocalyx Shedding and Microvascular Flow Abnormality in Mice and Swine. Int J Mol Sci 22:N/A (2021). PubMed: 34830227