Human ICAM1 ELISA Set (without plates) (ab47349)
Overview
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Product name
Human ICAM1 ELISA Set (without plates)
See all ICAM1 kits -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 0.1 ng/ml -
Range
0.25 ng/ml - 8 ng/ml -
Assay time
1h 45m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s ICAM1 Human in vitro ELISA Set is designed for the quantitative measurement of ICAM1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
This ELISA set is intended to measure human ICAM1 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
The ICAM1 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant human ICAM1.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests 15 x 96 tests Chromogen TMB Substrate Solution 4 vials 2 vials 6 vials Detection biotinylated anti-ICAM1 antibody 2 x 0.55ml 1 x 0.55ml 3 x 0.55ml ICAM1 Capture Antibody 2 x 0.5ml 1 x 0.5ml 3 x 0.5ml ICAM1 Standard 10 vials 5 vials 15 vials Streptavidin-HRP 2 vials 1 vial 3 vials -
Research areas
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Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsMonoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Antigen identified by monoclonal antibody BB2
- BB 2
- BB2
see all -
Database links
- Entrez Gene: 3383 Human
- Omim: 147840 Human
- SwissProt: P05362 Human
- Unigene: 643447 Human
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use at an assay dependent concentration.
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Notes |
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Sandwich ELISA
Use at an assay dependent concentration. |
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab47349 has been referenced in 3 publications.
- Meneses GC et al. Endothelial biomarkers as predictors for haemodialysis need in severe leptospirosis patients (Weil's disease). Trop Med Int Health 27:727-734 (2022). PubMed: 35761507
- Tessarolo LD et al. Endothelial activation is associated with albuminuria in multibacillary leprosy. Rev Inst Med Trop Sao Paulo 63:e44 (2021). PubMed: 34161550
- Lino DOC et al. Interleukin-6 and adhesion molecules VCAM-1 and ICAM-1 as biomarkers of post-acute myocardial infarction heart failure. Braz J Med Biol Res 52:e8658 (2019). PubMed: 31778438