Overview

  • Product nameAnti-ICAM2 antibody [3C4]
    See all ICAM2 primary antibodies
  • Description
    Rat monoclonal [3C4] to ICAM2
  • Tested applicationsSuitable for: IHC-FoFr, Flow Cyt, IHC-Fr, Blocking, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    COS cells transfected with Mouse ICAM2 cDNA.

  • General notes


    The 3C4 antibody blocks the interaction between ICAM2 and LFA1.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • Primary antibody notesThe 3C4 antibody blocks the interaction between ICAM2 and LFA1.
  • ClonalityMonoclonal
  • Clone number3C4
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/200.
Flow Cyt Use 1µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • RelevanceICAM2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin like C2 type domains, and bind to the leukocyte adhesion LFA1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA1 dependent cell adhesion. It mediates adhesive interactions important for antigen specific immune response, NK cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
  • Cellular localizationMembrane; Single-pass type I membrane protein
  • Database links
  • Alternative names
    • CD102 antibody
    • CD102 antigen antibody
    • ICAM 2 antibody
    • Intercellular adhesion molecule 2 antibody
    • Ly60 antibody
    see all

Anti-ICAM2 antibody [3C4] images

  • ab25246 staining ICAM2 in mouse olfactory epithelium tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with 1% paraformaldehyde + lysine + periodate (1% PLP), permeablized and blocked with 10% donkey serum + 5% nonfat dry milk + 4% BSA + 1% triton X-100 and antigen retrieval used 5% H2O2 in MeOH for 5 minutes room temperature. The sample was incubated with primary antibody (1/200) at 27°C for 1 hour. An Alexa Fluor® 488-conjugated donkey anti-rat polyclonal (1/150) was used as the secondary antibody.

    See Abreview

References for Anti-ICAM2 antibody [3C4] (ab25246)

ab25246 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step Other
Sample Mouse Tissue sections (Olfactory epithelium)
Specification Olfactory epithelium
Permeabilization Yes - .1% TritonX-100 in block
Fixative 1% Paraformaldehyde+lysine+periodate (1%PLP)
Username

Dr. Schwob Lab

Verified customer

Submitted Jun 11 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"