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Flow Cytometry / FACS - CD10 antibody [ICAM-2] (Biotin) (ab35268)
![Flow Cytometry / FACS - CD10 antibody [ICAM-2] (Biotin) (ab35268)](/ps/datasheet/images/ab35268.gif)
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Product Name
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ICAM2 antibody [CBR-IC2/2] (Biotin)
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See all ICAM2 antibodies (15)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [CBR-IC2/2] to ICAM2 (Biotin)
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Immunogen
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Transfected COS cells expressing ICAM 2 cDNA.
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Reacts with
(species key)
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Hu
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Specificity
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This antibody recognizes human CD10, also known as intercellular adhesion molecule 2 (ICAM 2).
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Tested applications
(see key)
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Flow Cyt
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Abreviews
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Application notes
(see key)
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Recommended dilutions Flow Cyt: 1/1. Use 10µl of neat antibody to label 106 cells in 100µl.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Type I membrane protein
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Research areas
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Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative Immunology >> Cell Type Markers >> CD >> Adhesion Immunology >> Adaptive Immunity >> B Cells >> CD
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Relevance
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ICAM2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin like C2 type domains, and bind to the leukocyte adhesion LFA1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA1 dependent cell adhesion. It mediates adhesive interactions important for antigen specific immune response, NK cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
Clone CBR-IC2/2 is reported to inhibit interactions between CD102 and LFA 1
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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CBR-IC2/2
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Myeloma
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P3x63-Ag8.653
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Isotype
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IgG2a
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Purification notes
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Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
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Form
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Liquid
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Conjugation
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Biotin
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ICAM2 antibody [CBR-IC2/2] (Biotin) is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ICAM2 antibody [CBR-IC2/2] (Biotin) images, references, products related to ab35268 and other tools.
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ICAM2 antibody [CBR-IC2/2] (Biotin) images:
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Flow Cytometry / FACS - CD10 antibody [ICAM-2] (Biotin) (ab35268)
ab35268 neat, staining peripheral blood monocytes in a FACS assay. 10µl of neat antibody to label 106 cells in 100µl.
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References for ICAM2 antibody [CBR-IC2/2] (Biotin) (ab35268)
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This clone has been used in: (two most recent references)
Issekutz AC et al. Role of ICAM-1 and ICAM-2 and alternate CD11/CD18 ligands in neutrophil transendothelial migration. J Leukoc Biol 65:117-26 (1999). PubMed: 9886254
Hart DN & Prickett TC Intercellular adhesion molecule-2 (ICAM-2) expression on human dendritic cells. Cell Immunol 148:447-54 (1993). PubMed: 8098673
See all 3 publication references for this product.
If you publish research using ab35268 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ICAM2
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ICAM2 antibody [CBR-IC2/2] (Biotin) - more information
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Customer reviews (feedback) regarding ICAM2 antibody [CBR-IC2/2] (Biotin) |
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Protocols for ICAM2 antibody [CBR-IC2/2] (Biotin) |
Price and availability of products related to ICAM2 antibody [CBR-IC2/2] (Biotin) |
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ICAM2 antibody [CBR-IC2/2] (Biotin) - related products:
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ICAM2 antibody [CBR-IC2/2] (Biotin) - other tools:
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Contact Abcam with a Technical Enquiry about ICAM2 antibody [CBR-IC2/2] (Biotin) |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"