Overview

  • Product nameAnti-ICAM3 antibody [EPR3994]
    See all ICAM3 primary antibodies
  • Description
    Rabbit monoclonal [EPR3994] to ICAM3
  • Tested applicationsSuitable for: ICC, WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in the extracellular domain of Human ICAM3.

  • Positive control
    • U937, Jurkat, HuT-78, Ramos, and THP-1 cell lysates; Human spleen, colon and tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab109405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.
    N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD50 antibody
    • CDW 50 antibody
    • CDw50 antibody
    • ICAM 3 antibody
    • ICAM-3 antibody
    • ICAM-R antibody
    • ICAM3 antibody
    • ICAM3_HUMAN antibody
    • ICAMR antibody
    • Intercellular adhesion molecule 3 antibody
    see all

Anti-ICAM3 antibody [EPR3994] images

  • All lanes : Anti-ICAM3 antibody [EPR3994] (ab109405) at 1/1000 dilution

    Lane 1 : U937 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HuT-78 cell lysate
    Lane 4 : Ramos cell lysate
    Lane 5 : THP-1 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 60 kDa
  • Immunohistochemical analysis of ICAM3 in paraffin-embedded Human spleen tissue using ab109405 at 1/500 dilution.
  • Immunohistochemical analysis of ICAM3 in paraffin-embedded Human colon tissue using ab109405 at 1/500 dilution.
  • Immunohistochemical analysis of ICAM3 in paraffin-embedded Human tonsil tissue using ab109405 at 1/500dilution.

References for Anti-ICAM3 antibody [EPR3994] (ab109405)

ab109405 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"