Recombinant
RabMAb

Anti-ICAM3 antibody [EPR3995] (ab108618)

Overview

  • Product name
    Anti-ICAM3 antibody [EPR3995]
    See all ICAM3 primary antibodies
  • Description
    Rabbit monoclonal [EPR3995] to ICAM3
  • Tested applications
    Suitable for: WB, IP, ICC, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of Human ICAM3.

  • Positive control
    • Jurkat, HL-60 and U937 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IP 1/10 - 1/100.
ICC 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.
    • Sequence similarities
      Belongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      modifications
      Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.
      N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD50 antibody
      • CDW 50 antibody
      • CDw50 antibody
      • ICAM 3 antibody
      • ICAM-3 antibody
      • ICAM-R antibody
      • ICAM3 antibody
      • ICAM3_HUMAN antibody
      • ICAMR antibody
      • Intercellular adhesion molecule 3 antibody
      see all

    Images

    • All lanes : Anti-ICAM3 antibody [EPR3995] (ab108618) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HL-60 cell lysate
      Lane 3 : U937 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 60 kDa

    References

    ab108618 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab108618.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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