Human ICAM3 ELISA Kit (CD50) (ab46024)
Key features and details
- Sensitivity: 0.3 ng/ml
- Range: 0.62 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ICAM3 ELISA Kit (CD50) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Sample A 8 19.75ng/ml 0.172 0.86% Sample B 8 2.83ng/ml 0.099 3.49% Inter-assay Sample n Mean SD CV% Sample A 36 19.51ng/ml 0.391 1.99% Sample B 36 2.71ng/ml 0.176 6.27% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 0.3 ng/ml -
Range
0.62 ng/ml - 20 ng/ml -
Assay time
1h 45m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s ICAM3 (CD50) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of ICAM3 in serum, plasma, buffered solutions and cell culture medium.
A monoclonal antibody specific for ICAM3 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known ICAM3 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for ICAM3 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of ICAM3 present in the samples.
This kit will recognize both endogenous and recombinant Human ICAM3.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Biotinylated anti-ICAM-3 (CD50) Red 1 x 0.4ml 2 x 0.4ml Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 12ml 1 x 23ml ICAM3 Control Silver 2 vials 4 vials ICAM3 Microplate (12 x 8 well strips) 1 unit 2 units ICAM3 Standard Yellow 2 vials 4 vials Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
Research areas
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Relevance
ICAM3 is a member of the intercellular adhesion molecule family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. ICAM3 is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. -
Cellular localization
Cell Membrane; single-pass type I membrane protein -
Alternative names
- CD50
- CDW50
- ICAM 3
see all -
Database links
- Entrez Gene: 3385 Human
- SwissProt: P32942 Human
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab46024 has not yet been referenced specifically in any publications.