Overview

  • Product name
  • Description
    Rabbit polyclonal to ICAM4
  • Tested applications
    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 43-210 of Human ICAM4 (BC000046).

  • Positive control
    • Human fetal colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab112554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins.
  • Tissue specificity
    Erythrocytes.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD242 antibody
    • CD242 antigen antibody
    • ICAM-4 antibody
    • Icam4 antibody
    • ICAM4_HUMAN antibody
    • Intercellular adhesion molecule 4 antibody
    • Landsteiner Wiener blood group glycoprotein antibody
    • Landsteiner-Wiener blood group glycoprotein antibody
    • LW antibody
    • LW blood group protein antibody
    see all

Images

  • ab112554, at a 1/100 dilution, staining ICAM4 in formalin-fixed, paraffin-embedded Human fetal colon tissue by Immunohistochemistry. Note: membrane staining.
  • Flow Cytometry analysis of ICAM4 in human endothelial precursor cells using ab112554 undiluted. The secondary used was an undiluted Allophycocyanin conjugated goat anti-rabbit polyclonal.
    Red line= ab112554
    Black line= isotype control

    See Abreview

References

This product has been referenced in:
  • Jackson AM  et al. Endothelial cell antibodies associated with novel targets and increased rejection. J Am Soc Nephrol 26:1161-71 (2015). Read more (PubMed: 25381426) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting Abcam.

Aswe carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which ...

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Thank you for contacting Abcam.

Both ab67943 and ab112554 are intact IgG molecules containing the Fc region. I have looked through our catalogue and I cannot find anything that matches your requirements, but you could try searching http://www...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Fixation
none
Sample
Human Cell (endothelial precursor cells)
Specification
endothelial precursor cells
Gating Strategy
CD133 and CD202B
Permeabilization
No
Username

Dr. A Jackson

Verified customer

Submitted Dec 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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