The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.03-0.06µg for 105-8 cells.
A final volume of 100 µL is recommended.
ab18542 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes.
Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus.
Staining of unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.03µg of Biotin-conjugated Rat IgG2b isotype control (open histogram) or 0.03µg of ab95632 (blue histogram) followed by Streptavidin PE. Viable cells were analyzed.