Anti-ICOS antibody [7E.17G9] - Low endotoxin, Azide free (ab185749)

Overview

  • Product name
    Anti-ICOS antibody [7E.17G9] - Low endotoxin, Azide free
    See all ICOS primary antibodies
  • Description
    Rat monoclonal [7E.17G9] to ICOS - Low endotoxin, Azide free
  • Host species
    Rat
  • Tested applications
    Suitable for: Flow Cyt, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • Con A activated (3 day) mouse splenocytes.
  • General notes

    Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by the LAL assay.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Functional Grade Purified.
  • Clonality
    Monoclonal
  • Clone number
    7E.17G9
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab185749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.125µg for 105-8 cells.

in 100μl volume.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Functional Studies Use at an assay dependent concentration.

inhibition of ligand binding

Target

  • Function
    Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes.
  • Tissue specificity
    Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus.
  • Involvement in disease
    Immunodeficiency, common variable, 1
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activation inducible lymphocyte immunomediatory molecule antibody
    • Activation-inducible lymphocyte immunomediatory molecule antibody
    • AILIM antibody
    • CD278 antibody
    • CD278 antigen antibody
    • CD28-related Protein-1 antibody
    • CRP1 antibody
    • CVID1 antibody
    • ICOS antibody
    • ICOS_HUMAN antibody
    • Inducible costimulator antibody
    • Inducible T cell co stimulator antibody
    • Inducible T-cell costimulator antibody
    • MGC39850 antibody
    see all

Images

  • Flow cytometric analysis of unstimulated (left) and 3 day ConA activated (right) BALB/c splenocytes labeling ICOS with 0.06 μg of Rat IgG2b Isotype Control (open histogram) or 0.06 μg of ab185749 (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.

References

ab185749 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab185749.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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