The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.125µg for 105-8 cells.
in 100μl volume.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
inhibition of ligand binding
Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes.
Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus.
Flow cytometric analysis of unstimulated (left) and 3 day ConA activated (right) BALB/c splenocytes labeling ICOS with 0.06 μg of Rat IgG2b Isotype Control (open histogram) or 0.06 μg of ab185749 (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.