• Product nameAnti-ICOS antibody [SP98]
    See all ICOS primary antibodies
  • Description
    Rabbit monoclonal [SP98] to ICOS
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to the C terminus of Human ICOS.

  • Positive control
    • Human Tonsil and Reactive Lymph Node
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.6
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified by protein A/G chromatography
  • ClonalityMonoclonal
  • Clone numberSP98
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab105227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at RT for 20 min.


  • FunctionEnhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes.
  • Tissue specificityActivated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus.
  • Involvement in diseaseImmunodeficiency, common variable, 1
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activation inducible lymphocyte immunomediatory molecule antibody
    • Activation-inducible lymphocyte immunomediatory molecule antibody
    • AILIM antibody
    • CD278 antibody
    • CD278 antigen antibody
    • CD28-related Protein-1 antibody
    • CRP1 antibody
    • CVID1 antibody
    • ICOS antibody
    • ICOS_HUMAN antibody
    • Inducible costimulator antibody
    • Inducible T cell co stimulator antibody
    • Inducible T-cell costimulator antibody
    • MGC39850 antibody
    see all

Anti-ICOS antibody [SP98] images

  • ab105227, at 1/100 dilution, staining ICOS in formalin-fixed, paraffin-embedded Human reactive lymph node tissue by Immunohistochemistry.

References for Anti-ICOS antibody [SP98] (ab105227)

This product has been referenced in:
  • Le KS  et al. Follicular B Lymphomas Generate Regulatory T Cells via the ICOS/ICOSL Pathway and Are Susceptible to Treatment by Anti-ICOS/ICOSL Therapy. Cancer Res 76:4648-60 (2016). Read more (PubMed: 27246829) »
  • Song Y  et al. Increased frequency of thymic T follicular helper cells in myasthenia gravis patients with thymoma. J Thorac Dis 8:314-22 (2016). IHC-P ; Human . Read more (PubMed: 27076925) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Spleen)
Specification Spleen
Fixative Acetone
Permeabilization No
Blocking step Casein as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C

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Verified customer

Submitted May 29 2012

Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab105227 in IHC-Fr and mouse. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offe...

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