Recombinant
RabMAb

Anti-ICOS Ligand antibody [EPR6071] (ab124972)

Overview

  • Product name
    Anti-ICOS Ligand antibody [EPR6071]
    See all ICOS Ligand primary antibodies
  • Description
    Rabbit monoclonal [EPR6071] to ICOS Ligand
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in the intracellular domain of Human ICOS Ligand.

  • Positive control
    • HL60, HuT78 and HUVEC (untreated or treated with TNF-alpha) lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 8.80 x 10 -11 M
    Learn more about KD
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR6071
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 63 - 72 kDa (predicted molecular weight: 33 kDa).
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.
    • Tissue specificity
      Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells.
    • Sequence similarities
      Belongs to the immunoglobulin superfamily. BTN/MOG family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • B7 H2 antibody
      • B7 homolog 2 antibody
      • B7 homologue 2 antibody
      • B7 like protein Gl50 antibody
      • B7 related protein 1 antibody
      • B7-H2 antibody
      • B7-like protein Gl50 antibody
      • B7-related protein 1 antibody
      • B7H2 antibody
      • B7RP 1 antibody
      • B7RP-1 antibody
      • B7RP1 antibody
      • CD 275 antibody
      • CD275 antibody
      • CD275 antigen antibody
      • GL 50 antibody
      • GL50 antibody
      • ICOS L antibody
      • ICOS LG antibody
      • ICOS ligand antibody
      • ICOSL antibody
      • ICOSL_HUMAN antibody
      • Icoslg antibody
      • Inducible T cell co stimulator ligand antibody
      • KIAA0653 antibody
      • LICOS antibody
      • Transmembrane protein B7 H2 ICOS ligand antibody
      see all

    Images

    • All lanes : Anti-ICOS Ligand antibody [EPR6071] (ab124972) at 1/1000 dilution

      Lane 1 : HL60 cell lysate
      Lane 2 : HuT78 cell lysate
      Lane 3 : HUVEC cell lysate, treated with TNF-alpha
      Lane 4 : HUVEC cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 33 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab124972 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab124972.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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