Anti-ICOS Ligand antibody [EPR6071] (ab124972)

Overview

  • Product nameAnti-ICOS Ligand antibody [EPR6071]
    See all ICOS Ligand primary antibodies
  • Description
    Rabbit monoclonal [EPR6071] to ICOS Ligand
  • Tested applicationsSuitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in the intracellular domain of Human ICOS Ligand.

  • Positive control
    • HL60, HuT78 and HUVEC (untreated or treated with TNF-alpha) lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD) KD = 8.80 x 10 -11 M Learn more about KD
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR6071
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 63 - 72 kDa (predicted molecular weight: 33 kDa).
ICC/IF 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • FunctionLigand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.
    • Tissue specificityIsoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells.
    • Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • B7 H2 antibody
      • B7 homolog 2 antibody
      • B7 homologue 2 antibody
      • B7 like protein Gl50 antibody
      • B7 related protein 1 antibody
      • B7-H2 antibody
      • B7-like protein Gl50 antibody
      • B7-related protein 1 antibody
      • B7H2 antibody
      • B7RP 1 antibody
      • B7RP-1 antibody
      • B7RP1 antibody
      • CD 275 antibody
      • CD275 antibody
      • CD275 antigen antibody
      • GL 50 antibody
      • GL50 antibody
      • ICOS L antibody
      • ICOS LG antibody
      • ICOS ligand antibody
      • ICOSL antibody
      • ICOSL_HUMAN antibody
      • Icoslg antibody
      • Inducible T cell co stimulator ligand antibody
      • KIAA0653 antibody
      • LICOS antibody
      • Transmembrane protein B7 H2 ICOS ligand antibody
      see all

    Anti-ICOS Ligand antibody [EPR6071] images

    • All lanes : Anti-ICOS Ligand antibody [EPR6071] (ab124972) at 1/1000 dilution

      Lane 1 : HL60 cell lysate
      Lane 2 : HuT78 cell lysate
      Lane 3 : HUVEC cell lysate, treated with TNF-alpha
      Lane 4 : HUVEC cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 33 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-ICOS Ligand antibody [EPR6071] (ab124972)

    ab124972 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab124972.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"