Overview

  • Product name
    Anti-Id1 antibody [2456C1a]
    See all Id1 primary antibodies
  • Description
    Mouse monoclonal [2456C1a] to Id1
  • Tested applications
    Suitable for: IHC-P, WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment from the internal region of human Id1

Properties

Applications

Our Abpromise guarantee covers the use of ab66495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
Dot blot
  • Application notes
    Dot: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 16 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.
    • Sequence similarities
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Developmental stage
      Expression correlates with proliferation in some types of cells.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • bHLHb24 antibody
      • Class B basic helix-loop-helix protein 24 antibody
      • dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein) antibody
      • DNA binding protein inhibitor ID 1 antibody
      • DNA binding protein inhibitor ID1 antibody
      • DNA-binding protein inhibitor ID-1 antibody
      • Dominant negative helix loop helix protein antibody
      • ID 1 antibody
      • ID antibody
      • ID1 antibody
      • ID1_HUMAN antibody
      • Inhibitor of Differentiation 1 antibody
      • Inhibitor of DNA binding 1 antibody
      • inhibitor of DNA binding 1, dominant negative helix-loop-helix protein antibody
      see all

    Images

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    ab66495 has not yet been tested in mouse samples, but based on the 95% sequence homology between the immunogen and mouse Id1, we would expect it to cross react.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (breast, prostate)
    Specification
    breast, prostate
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA
    Permeabilization
    No
    Username

    Abcam user community

    Verified customer

    Submitted Apr 02 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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