Overview

  • Product nameAnti-ID2 antibody [10C3]
    See all ID2 primary antibodies
  • Description
    Mouse monoclonal [10C3] to ID2
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human ID2 protein, expressed in E.coli.

  • Positive control
    • IHC-P: Human liver tissue and carcinoma of Human liver tissue ICC/IF: ID2 transfected HeLa cells
  • General notesDilute in PBS (pH 7.3) before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab90055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 15 kDa.
IHC-P 1/50.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • FunctionID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
  • Tissue specificityHighly expressed in early fetal tissues, including those of the central nervous system.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Developmental stageFound in most early fetal tissues but not in the corresponding mature tissues.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb26 antibody
    • Cell growth inhibiting gene 8 antibody
    • class B basic helix loop helix protein 26 antibody
    • Class B basic helix-loop-helix protein 26 antibody
    • DNA binding protein inhibitor ID 2 antibody
    • DNA binding protein inhibitor ID2 antibody
    • DNA-binding protein inhibitor ID-2 antibody
    • GIG 8 antibody
    • GIG8 antibody
    • Helix loop helix protein ID2 antibody
    • ID2 antibody
    • ID2_HUMAN antibody
    • ID2A antibody
    • ID2H antibody
    • Inhibitor of differentiation 2 antibody
    • Inhibitor of DNA binding 2 antibody
    • Inhibitor of DNA binding 2, dominant negative helix loop helix protein antibody
    • MGC26389 antibody
    see all

Anti-ID2 antibody [10C3] images

  • ab90055, at a dilution of 1/50, staining ID2 in paraffin embedded Human liver tissue by Immunohistochemistry.
  • ab90055, at a dilution of 1/50, staining ID2 in paraffin embedded carcinoma of Human liver tissue by Immunohistochemistry.
  • ab90055, staining ID2 in transiently transfected HeLa cells by Immunofluorescence.
  • Overlay histogram showing SH-SY5Y cells stained with ab90055 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90055, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-ID2 antibody [10C3] (ab90055)

ab90055 has not yet been referenced specifically in any publications.

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