Anti-ID2 antibody [2457C5a] (ab53545)

Overview

  • Product nameAnti-ID2 antibody [2457C5a]
    See all ID2 primary antibodies
  • Description
    Mouse monoclonal [2457C5a] to ID2
  • Tested applicationsSuitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human ID2

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS (8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4)
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab53545 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • ClonalityMonoclonal
  • Clone number2457C5a
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 32 kDa (predicted molecular weight: 15 kDa).
Dot blot Use at an assay dependent dilution.

Target

  • FunctionID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
  • Tissue specificityHighly expressed in early fetal tissues, including those of the central nervous system.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Developmental stageFound in most early fetal tissues but not in the corresponding mature tissues.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb26 antibody
    • Cell growth inhibiting gene 8 antibody
    • class B basic helix loop helix protein 26 antibody
    • Class B basic helix-loop-helix protein 26 antibody
    • DNA binding protein inhibitor ID 2 antibody
    • DNA binding protein inhibitor ID2 antibody
    • DNA-binding protein inhibitor ID-2 antibody
    • GIG 8 antibody
    • GIG8 antibody
    • Helix loop helix protein ID2 antibody
    • ID2 antibody
    • ID2_HUMAN antibody
    • ID2A antibody
    • ID2H antibody
    • Inhibitor of differentiation 2 antibody
    • Inhibitor of DNA binding 2 antibody
    • Inhibitor of DNA binding 2, dominant negative helix loop helix protein antibody
    • MGC26389 antibody
    see all

Anti-ID2 antibody [2457C5a] images

References for Anti-ID2 antibody [2457C5a] (ab53545)

ab53545 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Tissue lysate - other (rat spinal cord)
Loading amount 10 µg
Specification rat spinal cord
Gel Running Conditions Reduced Denaturing (gel 10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Minjung Kwon

Verified customer

Submitted Jan 05 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"