Overview

  • Product name
    Anti-ID2 antibody [4E12G5]
    See all ID2 primary antibodies
  • Description
    Mouse monoclonal [4E12G5] to ID2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Chimpanzee, Cynomolgus monkey, Orangutan
  • Immunogen

    Purified recombinant full length protein, corresponding to amino acids 1-134 of Human ID2, expressed in E. coli (UniProt: Q02363).

  • Positive control
    • Human recombinant ID2 protein; SK N SH cells; Human breast cancer and rectum cancer tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab166708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 17.3 kDa.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
  • Tissue specificity
    Highly expressed in early fetal tissues, including those of the central nervous system.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Developmental stage
    Found in most early fetal tissues but not in the corresponding mature tissues.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb26 antibody
    • Cell growth inhibiting gene 8 antibody
    • class B basic helix loop helix protein 26 antibody
    • Class B basic helix-loop-helix protein 26 antibody
    • DNA binding protein inhibitor ID 2 antibody
    • DNA binding protein inhibitor ID2 antibody
    • DNA-binding protein inhibitor ID-2 antibody
    • GIG 8 antibody
    • GIG8 antibody
    • Helix loop helix protein ID2 antibody
    • ID2 antibody
    • ID2_HUMAN antibody
    • ID2A antibody
    • ID2H antibody
    • Inhibitor of differentiation 2 antibody
    • Inhibitor of DNA binding 2 antibody
    • Inhibitor of DNA binding 2, dominant negative helix loop helix protein antibody
    • MGC26389 antibody
    see all

Images

  • Anti-ID2 antibody [4E12G5] (ab166708) at 1/500 dilution + recombinant Human ID2 protein at 1 µg

    Predicted band size: 17.3 kDa

  • Flow cytometric analysis of ID2-labeled SK N SH cells (green) and negative control cells (red), using ab166708 at a 1/200 dilution.
  • Immunohistochemical analysis of paraffin embedded, DAB-stained Human breast cancer tissue labeling ID2 using ab166708 at a 1/200 dilution.
  • Immunohistochemical analysis of paraffin embedded, DAB-stained Human rectum cancer tissue labeling ID2 using ab166708 at a 1/200 dilution.

References

This product has been referenced in:
  • Feng Y  et al. Alpha-1-antitrypsin functions as a protective factor in preeclampsia through activating Smad2 and inhibitor of DNA binding 4. Oncotarget 8:113002-113012 (2017). Read more (PubMed: 29348884) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA (pH 9.0)
Permeabilization
No
Specification
Mammary gland
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Jinwoo Seong

Verified customer

Submitted Jul 11 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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