Anti-ID2 antibody [4E12G5] (ab166708)
Key features and details
- Mouse monoclonal [4E12G5] to ID2
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-ID2 antibody [4E12G5]
See all ID2 primary antibodies -
Description
Mouse monoclonal [4E12G5] to ID2 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Chimpanzee, Cynomolgus monkey, Orangutan -
Immunogen
Purified recombinant full length protein, corresponding to amino acids 1-134 of Human ID2, expressed in E. coli (UniProt: Q02363).
-
Positive control
- Human recombinant ID2 protein; SK N SH cells; Human breast cancer and rectum cancer tissues.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4E12G5 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 17.3 kDa.
|
|
IHC-P | (1) |
1/200 - 1/1000.
|
Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
---|
WB
1/500 - 1/2000. Predicted molecular weight: 17.3 kDa. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
-
Function
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression. -
Tissue specificity
Highly expressed in early fetal tissues, including those of the central nervous system. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Developmental stage
Found in most early fetal tissues but not in the corresponding mature tissues. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 505025 Cow
- Entrez Gene: 3398 Human
- Entrez Gene: 15902 Mouse
- Entrez Gene: 654298 Pig
- Entrez Gene: 25587 Rat
- Omim: 600386 Human
- SwissProt: Q3ZC46 Cow
- SwissProt: Q4R5J7 Cynomolgus monkey
see all -
Alternative names
- bHLHb26 antibody
- Cell growth inhibiting gene 8 antibody
- class B basic helix loop helix protein 26 antibody
see all
Images
-
Immunohistochemical analysis of paraffin embedded, DAB-stained Human breast cancer tissue labeling ID2 using ab166708 at a 1/200 dilution.
-
Anti-ID2 antibody [4E12G5] (ab166708) at 1/500 dilution + recombinant Human ID2 protein at 1 µg
Predicted band size: 17.3 kDa -
Flow cytometric analysis of ID2-labeled SK N SH cells (green) and negative control cells (red), using ab166708 at a 1/200 dilution.
-
Immunohistochemical analysis of paraffin embedded, DAB-stained Human rectum cancer tissue labeling ID2 using ab166708 at a 1/200 dilution.
Protocols
Datasheets and documents
-
Datasheet download
References (8)
ab166708 has been referenced in 8 publications.
- Chen JT et al. Id2 exerts tumor suppressor properties in lung cancer through its effects on cancer cell invasion and migration. Front Oncol 12:801300 (2022). PubMed: 35982951
- Yuan M et al. Phospholipase C (PLC)e Promotes Androgen Receptor Antagonist Resistance via the Bone Morphogenetic Protein (BMP)-6/SMAD Axis in a Castration-Resistant Prostate Cancer Cell Line. Med Sci Monit 25:4438-4449 (2019). PubMed: 31201297
- Liu T et al. miR-27a promotes endothelial-mesenchymal transition in hypoxia-induced pulmonary arterial hypertension by suppressing BMP signaling. Life Sci 227:64-73 (2019). PubMed: 31004656
- Fan H et al. Effect of Notch1 gene on remyelination in multiple sclerosis in mouse models of acute demyelination. J Cell Biochem N/A:N/A (2018). PubMed: 30010211
- Hwang SM et al. Inflammation-induced Id2 promotes plasticity in regulatory T cells. Nat Commun 9:4736 (2018). PubMed: 30413714
- Le Dréau G et al. E proteins sharpen neurogenesis by modulating proneural bHLH transcription factors' activity in an E-box-dependent manner. Elife 7:N/A (2018). PubMed: 30095408
- Feng Y et al. Alpha-1-antitrypsin functions as a protective factor in preeclampsia through activating Smad2 and inhibitor of DNA binding 4. Oncotarget 8:113002-113012 (2017). PubMed: 29348884
- Wang S et al. Regulatory Innate Lymphoid Cells Control Innate Intestinal Inflammation. Cell 171:201-216.e18 (2017). PubMed: 28844693