Overview

  • Product name
  • Description
    Rabbit polyclonal to ID2
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide from C-terminal of human ID2 protein

  • Positive control
    • Breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab52093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
  • Application notes
    IHC-P: 1/50 for 30 min at RT.
    Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
    • Tissue specificity
      Highly expressed in early fetal tissues, including those of the central nervous system.
    • Sequence similarities
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Developmental stage
      Found in most early fetal tissues but not in the corresponding mature tissues.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • bHLHb26 antibody
      • Cell growth inhibiting gene 8 antibody
      • class B basic helix loop helix protein 26 antibody
      • Class B basic helix-loop-helix protein 26 antibody
      • DNA binding protein inhibitor ID 2 antibody
      • DNA binding protein inhibitor ID2 antibody
      • DNA-binding protein inhibitor ID-2 antibody
      • GIG 8 antibody
      • GIG8 antibody
      • Helix loop helix protein ID2 antibody
      • ID2 antibody
      • ID2_HUMAN antibody
      • ID2A antibody
      • ID2H antibody
      • Inhibitor of differentiation 2 antibody
      • Inhibitor of DNA binding 2 antibody
      • Inhibitor of DNA binding 2, dominant negative helix loop helix protein antibody
      • MGC26389 antibody
      see all

    Anti-ID2 antibody images

    • Staining of ID2 in human breast carcinoma by immunohistochemistry, using ab52093 (1/50). (Formalin fixed, Paraffin embedded section).

    References for Anti-ID2 antibody (ab52093)

    ab52093 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Human embryonic lung - 7 weeks post conception)
    Permeabilization
    Yes - 10 min 0.3% triton post-sectioning
    Specification
    Human embryonic lung - 7 weeks post conception
    Blocking step
    1% BSA, 5% normal donkey serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Username

    Ms. Emma Rawlins

    Verified customer

    Submitted May 24 2017

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Sample
    Mouse Cell (bEnd.3)
    Specification
    bEnd.3
    Permeabilization
    Yes - 0.1% Triton X100
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 14 2014

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Sample
    Human Cell (HUVEC)
    Specification
    HUVEC
    Permeabilization
    Yes - 0.1% Triton X100
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 02 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (breast cancer, prostate cancer)
    Specification
    breast cancer, prostate cancer
    Fixative
    Formaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Username

    Abcam user community

    Verified customer

    Submitted Feb 09 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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