Overview

  • Product nameAnti-ID2 antibody
    See all ID2 primary antibodies
  • Description
    Rabbit polyclonal to ID2
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide from C-terminal of human ID2 protein

  • Positive control
    • Breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab52093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
  • Application notesIHC-P: 1/50 for 30 min at RT.
    Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
    • Tissue specificityHighly expressed in early fetal tissues, including those of the central nervous system.
    • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
    • Developmental stageFound in most early fetal tissues but not in the corresponding mature tissues.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • bHLHb26 antibody
      • Cell growth inhibiting gene 8 antibody
      • class B basic helix loop helix protein 26 antibody
      • Class B basic helix-loop-helix protein 26 antibody
      • DNA binding protein inhibitor ID 2 antibody
      • DNA binding protein inhibitor ID2 antibody
      • DNA-binding protein inhibitor ID-2 antibody
      • GIG 8 antibody
      • GIG8 antibody
      • Helix loop helix protein ID2 antibody
      • ID2 antibody
      • ID2_HUMAN antibody
      • ID2A antibody
      • ID2H antibody
      • Inhibitor of differentiation 2 antibody
      • Inhibitor of DNA binding 2 antibody
      • Inhibitor of DNA binding 2, dominant negative helix loop helix protein antibody
      • MGC26389 antibody
      see all

    Anti-ID2 antibody images

    • Staining of ID2 in human breast carcinoma by immunohistochemistry, using ab52093 (1/50). (Formalin fixed, Paraffin embedded section).

    References for Anti-ID2 antibody (ab52093)

    ab52093 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Sample Mouse Cell (bEnd.3)
    Specification bEnd.3
    Permeabilization Yes - 0.1% Triton X100
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 14 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Sample Human Cell (HUVEC)
    Specification HUVEC
    Permeabilization Yes - 0.1% Triton X100
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 02 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (breast cancer, prostate cancer)
    Specification breast cancer, prostate cancer
    Fixative Formaldehyde
    Antigen retrieval step None
    Permeabilization No
    Username

    Abcam user community

    Verified customer

    Submitted Feb 09 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"