Overview

  • Product nameAnti-ID2 antibody
    See all ID2 primary antibodies
  • Description
    Rabbit polyclonal to ID2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 85-134 (IVSLHHQRPG QNQASRTPLT TLNTDISILS LQASEFPSEL MSNDSKALCG) of human ID2 (NP_002157).

  • Positive control
    • Human fetal thymus nuclear lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab87189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 15 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
  • Tissue specificityHighly expressed in early fetal tissues, including those of the central nervous system.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Developmental stageFound in most early fetal tissues but not in the corresponding mature tissues.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb26 antibody
    • Cell growth inhibiting gene 8 antibody
    • class B basic helix loop helix protein 26 antibody
    • Class B basic helix-loop-helix protein 26 antibody
    • DNA binding protein inhibitor ID 2 antibody
    • DNA binding protein inhibitor ID2 antibody
    • DNA-binding protein inhibitor ID-2 antibody
    • GIG 8 antibody
    • GIG8 antibody
    • Helix loop helix protein ID2 antibody
    • ID2 antibody
    • ID2_HUMAN antibody
    • ID2A antibody
    • ID2H antibody
    • Inhibitor of differentiation 2 antibody
    • Inhibitor of DNA binding 2 antibody
    • Inhibitor of DNA binding 2, dominant negative helix loop helix protein antibody
    • MGC26389 antibody
    see all

Anti-ID2 antibody images

  • Anti-ID2 antibody (ab87189) at 1 µg/ml + human fetal thymus lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 15 kDa
    Observed band size : 15 kDa

References for Anti-ID2 antibody (ab87189)

This product has been referenced in:
  • Tamaki S & Tokumoto Y Overexpression of cyclin dependent kinase inhibitor P27/Kip1 increases oligodendrocyte differentiation from induced pluripotent stem cells. In Vitro Cell Dev Biol Anim 50:778-85 (2014). WB ; Mouse . Read more (PubMed: 24764126) »

See 1 Publication for this product

Product Wall

Abreviews
Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing
Sample Rat Cell lysate - whole cell (glial progenitors)
Specification glial progenitors
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 10 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"