Overview

  • Product nameAnti-ID2 antibody
    See all ID2 primary antibodies
  • Description
    Mouse monoclonal to ID2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human ID2 protein (AAH30639) with a 26 kDa tag

  • Positive control
    • HeLa cell lysate, HepG2 cell lysate and ID2 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab89530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 15 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
  • Tissue specificityHighly expressed in early fetal tissues, including those of the central nervous system.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Developmental stageFound in most early fetal tissues but not in the corresponding mature tissues.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb26 antibody
    • Cell growth inhibiting gene 8 antibody
    • class B basic helix loop helix protein 26 antibody
    • Class B basic helix-loop-helix protein 26 antibody
    • DNA binding protein inhibitor ID 2 antibody
    • DNA binding protein inhibitor ID2 antibody
    • DNA-binding protein inhibitor ID-2 antibody
    • GIG 8 antibody
    • GIG8 antibody
    • Helix loop helix protein ID2 antibody
    • ID2 antibody
    • ID2_HUMAN antibody
    • ID2A antibody
    • ID2H antibody
    • Inhibitor of differentiation 2 antibody
    • Inhibitor of DNA binding 2 antibody
    • Inhibitor of DNA binding 2, dominant negative helix loop helix protein antibody
    • MGC26389 antibody
    see all

Anti-ID2 antibody images

  • Anti-ID2 antibody (ab89530) at 5 µg/ml + recombinant protein (the immunogen) at 0.2 µg

    Predicted band size : 15 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
  • Anti-ID2 antibody (ab89530) at 5 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 15 kDa
    Observed band size : 15 kDa
  • Anti-ID2 antibody (ab89530) at 5 µg/ml + HepG2 cell lysate at 50 µg

    Predicted band size : 15 kDa
    Observed band size : 15 kDa
  • All lanes : Anti-ID2 antibody (ab89530) at 5 µg/ml

    Lane 1 : ID2 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 15 kDa
    Observed band size : 15 kDa

References for Anti-ID2 antibody (ab89530)

ab89530 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab89530.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"