ID2 overexpression 293T lysate (whole cell) (ab94214)

Overview

  • Product nameID2 overexpression 293T lysate (whole cell)
  • Description
    ID2 overexpression lysate
  • General notesab94214 is a 293T cell transfected lysate in which Human ID2 has been transiently over-expressed using a pCMV-ID2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundDevelopmental stage: Found in most early fetal tissues but not in the corresponding mature tissues. Function: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. Tissue specificity: Highly expressed in early fetal tissues, including those of the central nervous system.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

ID2 overexpression 293T lysate (whole cell) images

  • ab94214 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-ID2 antibody (ab89530) at 1/500 dilution

    Lane 1 : ID2 overexpression 293T lysate (whole cell) (ab94214)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution

References for ID2 overexpression 293T lysate (whole cell) (ab94214)

ab94214 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"