Anti-ID3 antibody [10D3] (ab118032)

Overview

  • Product nameAnti-ID3 antibody [10D3]
    See all ID3 primary antibodies
  • Description
    Mouse monoclonal [10D3] to ID3
  • Tested applicationsSuitable for: IHC-Fr, WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Zebrafish
  • Immunogen

    Recombinant full length Human ID3 produced in HEK293T cells (NP_002158).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY ID3 cDNA; Human liver tissue; HeLa and Jurkat cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab118032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.
WB 1/500. Predicted molecular weight: 13 kDa.
IHC-P 1/50.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors.
  • Tissue specificityExpressed abundantly in lung, kidney and adrenal gland, but not in adult brain.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1R21 antibody
    • bHLHb25 antibody
    • Class B basic helix-loop-helix protein 25 antibody
    • DNA binding protein inhibitor ID 3 antibody
    • DNA-binding protein inhibitor ID-3 antibody
    • HEIR 1 antibody
    • HEIR1 antibody
    • Helix loop helix protein HEIR 1 antibody
    • Helix loop helix protein HEIR1 antibody
    • Helix-loop-helix protein HEIR-1 antibody
    • HLH642 antibody
    • ID 3 antibody
    • ID like protein inhibitor HLH 1R21 antibody
    • ID like protein inhibitor HLH 462 antibody
    • ID-like protein inhibitor HLH 1R21 antibody
    • ID3 antibody
    • ID3_HUMAN antibody
    • Inhibitor of DNA binding 3 antibody
    • Inhibitor of DNA binding 3 dominant negative helix loop helix protein antibody
    see all

Anti-ID3 antibody [10D3] images

  • ab118032 staining ID3 in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab118032 at 1/100 in blocking solution for 16h at 4°C. An alexa 647 conjugated goat anti-mouset polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. ID3 expression can be observed in Muller cells.

    See Abreview

  • All lanes : Anti-ID3 antibody [10D3] (ab118032) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY ID3 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 13 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200583)
  • ab118032 at 1/50 dilution staining ID3 in Paraffin-embedded Human Liver tissue by Immunohistochemistry.
  • ab118032 at 1/100 dilution staining ID3 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
  • ab118032 at 1/100 dilution staining ID3 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

References for Anti-ID3 antibody [10D3] (ab118032)

ab118032 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (Retina, Muller Cell)
Specification Retina, Muller Cell
Permeabilization Yes - 0.1% Triton X
Fixative Paraformaldehyde
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Submitted Nov 07 2013

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