- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-ID3 antibody
See all ID3 primary antibodies -
DescriptionRabbit polyclonal to ID3
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-Fr, ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide corresponding to Human ID3 (C terminal).
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Positive control
- Human breast carcinoma.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4 -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab41834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. PubMed: 20460439 | |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IHC-P | 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors.
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Tissue specificityExpressed abundantly in lung, kidney and adrenal gland, but not in adult brain.
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Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 3399 Human
- Entrez Gene: 15903 Mouse
- Omim: 600277 Human
- SwissProt: Q02535 Human
- SwissProt: P41133 Mouse
- Unigene: 76884 Human
- Unigene: 110 Mouse
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Alternative names
- 1R21 antibody
- bHLHb25 antibody
- Class B basic helix-loop-helix protein 25 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody (ab41834)Ab41834 staining human ID3 in human breast carcinoma by immunohistochemistry using formalin fixed, paraffin embedded tissue. -
Immunocytochemistry/ Immunofluorescence - Anti-ID3 antibody (ab41834)ICC/IF image of ab41834 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41834, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References
This product has been referenced in:
- Forni PE et al. The indirect role of fibroblast growth factor-8 in defining neurogenic niches of the olfactory/GnRH systems. J Neurosci 33:19620-34 (2013). Mouse . Read more (PubMed: 24336726) »
- Snyder AD et al. Expression pattern of id proteins in medulloblastoma. Pathol Oncol Res 19:437-46 (2013). Read more (PubMed: 23397264) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"