Customer reviews and FAQs

Overview

  • Product name
    Anti-ID3 antibody
    See all ID3 primary antibodies
  • Description
    Rabbit polyclonal to ID3
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human ID3 (C terminal).

  • Positive control
    • Human breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab41834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 20460439
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors.
  • Tissue specificity
    Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • 1R21 antibody
      • bHLHb25 antibody
      • Class B basic helix-loop-helix protein 25 antibody
      • DNA binding protein inhibitor ID 3 antibody
      • DNA-binding protein inhibitor ID-3 antibody
      • HEIR 1 antibody
      • HEIR1 antibody
      • Helix loop helix protein HEIR 1 antibody
      • Helix loop helix protein HEIR1 antibody
      • Helix-loop-helix protein HEIR-1 antibody
      • HLH642 antibody
      • ID 3 antibody
      • ID like protein inhibitor HLH 1R21 antibody
      • ID like protein inhibitor HLH 462 antibody
      • ID-like protein inhibitor HLH 1R21 antibody
      • ID3 antibody
      • ID3_HUMAN antibody
      • Inhibitor of DNA binding 3 antibody
      • Inhibitor of DNA binding 3 dominant negative helix loop helix protein antibody
      see all

    Anti-ID3 antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ID3 antibody (ab41834)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ID3 antibody (ab41834)
      Ab41834 staining human ID3 in human breast carcinoma by immunohistochemistry using formalin fixed, paraffin embedded tissue.
    • Immunocytochemistry/ Immunofluorescence-Anti-ID3 antibody(ab41834)
      Immunocytochemistry/ Immunofluorescence-Anti-ID3 antibody(ab41834)
      ICC/IF image of ab41834 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41834, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-ID3 antibody (ab41834)

    This product has been referenced in:
    • Forni PE  et al. The indirect role of fibroblast growth factor-8 in defining neurogenic niches of the olfactory/GnRH systems. J Neurosci 33:19620-34 (2013). Mouse . Read more (PubMed: 24336726) »
    • Gajera CR  et al. LRP2 in ependymal cells regulates BMP signaling in the adult neurogenic niche. J Cell Sci 123:1922-30 (2010). IHC-Fr ; Mouse . Read more (PubMed: 20460439) »

    See all 2 Publications for this product

    Publishing research using ab41834? Please let us know so that we can cite the reference in this datasheet.

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    Submit an abreview for ab41834 Submit a question for ab41834

    2 Abreviews or Q&A

    10
    Votes: Highest First

    I found another Id3 antibody on your website(ab41834) with reference paper on http://www.ncbi.nlm.nih.gov/pubmed/20460439?dopt=Abstract## I would like to have this antibody as the replacement for the IHC(mice tissue) on the last figure for a paper whic...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ID3 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (breast cancer, prostate cancer, kidney)
    Specification
    breast cancer, prostate cancer, kidney
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate, 20 minutes at 100C
    Permeabilization
    No
    Read More
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    Abcam user community

    Verified customer

    Submitted Feb 17 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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