Overview

  • Product nameAnti-ID3 antibody
    See all ID3 primary antibodies
  • Description
    Mouse monoclonal to ID3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MKALSPVRGC YEAVCCLSER SLAIARGRGK GPAAEEPLSL LDDMNHCYSR LRELVPGVPR GTQLSQVEIL QRVIDYILDL QVV, corresponding to amino acids 1-84 of Human ID3

Properties

Applications

Our Abpromise guarantee covers the use of ab55269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 13 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors.
  • Tissue specificityExpressed abundantly in lung, kidney and adrenal gland, but not in adult brain.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1R21 antibody
    • bHLHb25 antibody
    • Class B basic helix-loop-helix protein 25 antibody
    • DNA binding protein inhibitor ID 3 antibody
    • DNA-binding protein inhibitor ID-3 antibody
    • HEIR 1 antibody
    • HEIR1 antibody
    • Helix loop helix protein HEIR 1 antibody
    • Helix loop helix protein HEIR1 antibody
    • Helix-loop-helix protein HEIR-1 antibody
    • HLH642 antibody
    • ID 3 antibody
    • ID like protein inhibitor HLH 1R21 antibody
    • ID like protein inhibitor HLH 462 antibody
    • ID-like protein inhibitor HLH 1R21 antibody
    • ID3 antibody
    • ID3_HUMAN antibody
    • Inhibitor of DNA binding 3 antibody
    • Inhibitor of DNA binding 3 dominant negative helix loop helix protein antibody
    see all

Anti-ID3 antibody images



  • Predicted band size : 13 kDa
    ID3 antibody (ab55269) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab55269 at 10 ug/ml staining ID3 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-ID3 antibody (ab55269)

ab55269 has not yet been referenced specifically in any publications.

Product Wall

I'm happy to help. Please contact us again if you have any further questions.

All the testing data we have using ab55269 can be found on the online datasheet for ab55269 on our website. The tissues I noted as expressing high levels of ID3 was information I found in SwissProt database of proteins. We have links to this and other ...

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I have found that ID3 is expressed abundantly in lung, kidney and adrenal gland, but not in adult brain. Also, to increase sensitivity of detection, I would recommend using a higher concentration of antibody, such as 2 or 4ug/ml. Also, if you a...

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Thank you for your enquiry. I think it would be helpful to get a bit more information on your protocol and sample preparation methods. For example, are you doing western blotting or ICC? What dilution of the primary antibody are you using? What...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"