General notesab94230 is a 293T cell transfected lysate in which Human ID3 has been transiently over-expressed using a pCMV-ID3 plasmid. The lysate is provided in 1X Sample Buffer.
Note: For more details about how the transfected lysate was prepared view preparation notes
BackgroundFunction: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors.
Tissue specificity: Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain.
Similarity: Contains 1 basic helix-loop-helix (bHLH) domain.