Overview

  • Product nameAnti-ID4 antibody
    See all ID4 primary antibodies
  • Description
    Rabbit polyclonal to ID4
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Pig
  • Immunogen

    A reigon within synthetic peptide: MKAVSPVRPS GRKAPSGCGG GELALRCLAE HGHSLGGSAA AAAAAAAARC, corresponding to N terminal amino acids 1-50 of Human ID4

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab49261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 18 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration. PubMed: 21297112

Target

Anti-ID4 antibody images

  • Anti-ID4 antibody (ab49261) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG 1/50000-1/100000

    Predicted band size : 18 kDa
    Observed band size : 18 kDa

References for Anti-ID4 antibody (ab49261)

This product has been referenced in:
  • Phi JH  et al. ID3 contributes to cerebrospinal fluid seeding and poor prognosis in medulloblastoma. BMC Cancer 13:291 (2013). WB ; Human . Read more (PubMed: 23768125) »
  • Melcher R  et al. LOH and copy neutral LOH (cnLOH) act as alternative mechanism in sporadic colorectal cancers with chromosomal and microsatellite instability. Carcinogenesis 32:636-42 (2011). IHC-P ; Human . Read more (PubMed: 21297112) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Testis, postnatal day 6)
Permeabilization Yes - 0.1% Triton X-100 in PBS
Specification Testis, postnatal day 6
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

Submitted Sep 15 2016

Abreviews
Application Western blot
Sample Rat Tissue lysate - other (spinal cord)
Loading amount 10 µg
Specification spinal cord
Gel Running Conditions Reduced Denaturing (10%gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Minjung Kwon

Verified customer

Submitted Jan 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"