Overview

  • Product name
  • Description
    Goat polyclonal to IDH2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Horse, Chicken, Cow, Dog, Pig, Macaque monkey
  • Immunogen

    Synthetic peptide:

    CIHGLSNVKLNE

    , corresponding to internal sequence amino acids 418-429 of Human IDH2 according to NP_002159.2

  • Positive control
    • Human, Mouse or Rat Heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab109588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa).

Target

Images

  • All lanes : Anti-IDH2 antibody (ab109588) at 0.1 µg/ml

    Lane 1 : Mouse heart tissue lysates
    Lane 2 : Rat heart tissue lysates
    Lane 3 : Pig heart tissue lysates

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 51 kDa

    Primary incubation was 1 hour.

  • Anti-IDH2 antibody (ab109588) at 0.1 µg/ml + Human heart lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 51 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour.

References

ab109588 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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