Overview

  • Product name
  • Description
    Rabbit polyclonal to IDH2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Recombinant fragment within Human IDH2 aa 402-452. The exact sequence is proprietary. NP_002159.2
    Sequence:

    CVETVESGAMTKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDRALGR Q


    Database link: P48735

  • Positive control
    • Whole cell lysate from 293T, HeLa, Jurkat, mouse TCMK-1 and NIH3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab177512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/1000 - 1/5000. Predicted molecular weight: 51 kDa.

Target

  • Function
    Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
  • Involvement in disease
    D-2-hydroxyglutaric aciduria 2
    Glioma
    enetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma.
  • Sequence similarities
    Belongs to the isocitrate and isopropylmalate dehydrogenases family.
  • Post-translational
    modifications
    Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • D2HGA2 antibody
    • ICD-M antibody
    • IDH antibody
    • IDH2 antibody
    • IDHM antibody
    • IDHP_HUMAN antibody
    • IDP antibody
    • IDPM antibody
    • Isocitrate dehydrogenase [NADP], mitochondrial antibody
    • Isocitrate dehydrogenase 2 (NADP+), mitochondrial antibody
    • mNADP-IDH antibody
    • NADP(+)-specific ICDH antibody
    • Oxalosuccinate decarboxylase antibody
    see all

Images

  • All lanes : Anti-IDH2 antibody (ab177512) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from 293T cells
    Lane 2 : Whole cell lysate from HeLa cells
    Lane 3 : Whole cell lysate from Jurkat cells
    Lane 4 : Whole cell lysate from TCMK-1 cells
    Lane 5 : Whole cell lysate from NIH3T3 cells

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 51 kDa


    Exposure time: 30 seconds
  • Lane 1: ab177512 at 1µg/ml labeling IDH2 in 293T whole cell lysate immunoprecipitated using ab177512 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

    Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 10 seconds.

    .

References

ab177512 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab177512.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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